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Gel Electrophoresis
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JoVE Core
Biology
Education
Gel Electrophoresis
 

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Gel Electrophoresis

DNA gel electrophoresis is a technique used for the detection and separation of DNA molecules. An electric field is applied to a gel matrix comprised of agarose, and within the gel, charge particles will migrate and separate based on size. The negatively charged phosphates of the DNA backbone cause DNA fragments to move toward the anode—a positively charged electrode.

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