Jove Lab Chem

Lab 39: Buffers — Materials List

Preparation for 10 Students

• Lab stand
  5
• Thermometer clamp
  5
• pH 4.00 calibration buffer
  5
• pH 7.00 calibration buffer
  5
• Handheld data acquisition device
  5
• UV-Vis spectrophotometer attachment
  5
• pH probe attachment
  5
• Flash drive
  5
• 10-mL glass graduated cylinder
  10
• 50-mL glass graduated cylinder
  5
• 100-mL glass beaker
  10
• 400-mL glass beaker
  10
• 25-mL glass beaker
  5
• 1.5-mL cuvette with cap
  50
• Small watch glass
  5
• 250-mL plastic wash bottle
  5
• 250-mL polyethylene bottle with cap
  5
• 70-mm long-stem funnel
  5
• Base-resistant long forceps
  5
• Disposable plastic pipettes
  Dependent on lab size
• Stir plate
  5
• Medium stir bar
  5
• NaOH
  5 g
• Neutral red
  2 mg
• Riboflavin-binding protein
  200 mg
• Anhydrous NaH₂PO₄
  10 g
• 5-mL test tube
  6
• 20-mL glass or polyethylene vial with cap
  5
• 125-mL glass Erlenmeyer flask
  1
• 100-mL glass graduated cylinder
  1
• 125-mL polyethylene bottle
  8
• 100-mL polyethylene bottle
  1
• 25-mL glass graduated cylinder
  1
• Magnetic wand
  1
• 10-mL glass volumetric flask
  1
• 100-mL glass volumetric flask
  1
• Micro stir bar
  1
• Small test tube rack
  1
• Lab spatula
  Dependent on lab size
• Analytical balance (at least 1)
  Dependent on lab size
• Weighing paper
  Dependent on lab size
• Deionized water
  Dependent on lab size
• Plastic paraffin film
  Dependent on lab size
• 1-mL micropipette with tips (+8 for buffers)
  5
• 200-µL pipette with tips
  5
• Laboratory tape
  5 roll
• Laboratory wipes
  5 box
• 50-mM NaOAc buffer, pH 5.0
  50 mL
• 50-mM NaOAc buffer, pH 5.5
  50 mL
• 50-mM NaOAc buffer, pH 6.0
  50 mL
• 50-mM NaH₂PO₄ buffer, pH 6.5
  50 mL
• 50-mM Tris-HCl buffer, pH 7.5
  50 mL
• 50-mM Tris-HCl buffer, pH 8.0
  50 mL
• 50-mM Tris-HCl buffer, pH 8.5
  50 mL
• 25-mM Na₂HPO₄ buffer, pH 11
  50 mL