Jove Lab Chem
Extraction — Lab Prep
Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA
- Preparation of the Laboratory
Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.
-
Put on a lab coat, splash-proof safety glasses, and nitrile gloves.
-
Place labeled containers for halogenated waste and solid reagent waste in a satellite waste hood. Place a glass waste container nearby.
-
Label a 20-mL vial as ‘unknown mixture’.
-
Measure out 0.5 g of cellulose and add it to the labeled vial. Measure out 4.75 g each of benzoic acid and caffeine and add them to the vial. Stir the solids well with a glass rod.
-
Tightly cap the vial and place it by the balances. Set a box of 90-mm circular filter paper by the balances. Make sure that the area has enough weighing boats, laboratory wipes, and clean spatulas.
-
Set out the following glassware and equipment at each student lab station (we suggest that students work in pairs):
1 Lab stand with ring fixture 1 Hotplate 1 Vacuum pump (or house vacuum) 1 Medium 3-prong clamp 1 125-mL separatory funnel with a stopper 1 125-mL Erlenmeyer flask 3 10-mL glass graduated cylinders 2 50-mL glass graduated cylinders 2 50-mL beakers 3 100-mL beakers 1 600-mL beaker 1 250-mL filter flask 1 Büchner funnel 2 60-mL glass funnels 2 100-mm watch glasses 2 Glass stirring rods 2 24/40 rubber adapters 2 Pasteur pipette bulbs 2 Small spatulas 1 Package of pH paper 1 Roll of lab tape and pen -
Distribute boxes of lab wipes and Pasteur pipettes around the lab so that each workstation has them nearby.
-
Set out anhydrous magnesium sulfate, sodium bicarbonate, vacuum grease, and several spatulas on a common bench.
-
Shortly before the lab, place labeled bottles of dichloromethane and 1 M NaOH in a fume hood.
-
Put a bottle of 3 M HCl in a separate fume hood to avoid inadvertent contact between acids and bases.
-