Jove Lab Chem

Lab 57: Thin-Layer Chromatography — Materials List

Preparation for 10 Students

• 50-mL screw-top glass jar with solvent-resistant cap (inner diameter of 4 cm)
  15
• 10-mL glass graduated cylinder
  15
• Pencil
  5
• Labeling pen
  5
• Metal tweezers (10 or 12 cm)
  5
• Small ruler with mm and cm increments
  5
• Plastic/glass backed normal-phase silica gel analytic TLC plate with fluorescent indicator at 253 nm (2.5 cm x 6.5 cm)
  15
• Filter paper
  15
• Disposable glass capillary (+ extra)
  50
• Phenol
  20 mg
• Benzoic acid
  20 mg
• Butoxybenzene/butyl phenyl ether
  20 mg
• Acetylsalicylic acid/aspirin
  20 mg
• Caffeine
  20 mg
• Acetaminophen/paracetamol
  20 mg
• Ethanol (denatured)
  200 mL
• Hexane
  300 mL
• Ethyl acetate (98% HPLC grade)
  200 mL
• OTC tablet (325 mg acetaminophen)
  5
• OTC tablet (325 mg acetylsalicylic acid)
  5
• OTC tablet (250 mg acetaminophen, 250 mg acetylsalicylic acid, 65 mg caffeine)
  5
• Iodine crystals
  2 g
• Stirring hotplate
  1
• Mortar and pestle
  1
• 10-mL graduated cylinder
  1
• 100-mL graduated cylinder
  2
• 250- or 400-mL glass beaker
  2
• 150-mL glass bottle with solvent-resistant cap
  3
• 20-mL glass vials with solvent-resistant cap
  9
• Glass stirring rod
  4
• Glass funnel
  3
• 20- or 100-μL micropipette with pipette tips
  1
• 500-mL glass beaker
  1
• 100-mm watch glass
  1
• Analytical balance
  1
• Weighing boats
  5
• Spatulas
  3
• Labeling tape
  1 roll
• Lab wipes
  Dependent on lab size
• 254-nm UV lamp (at least 1)
  Dependent on lab size