Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

7.7: DNA Damage can Stall the Cell Cycle
TABLE OF
CONTENTS

JoVE Core
Molecular Biology

A subscription to JoVE is required to view this content. You will only be able to see the first 20 seconds.

Education
DNA Damage can Stall the Cell Cycle
 
TRANSCRIPT

7.7: DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at the right time for damage response in a tightly regulated cell cycle.

Replication stress caused by damaged DNA initiates a carefully choreographed pathway of proteins that respond to the specific type of damage with an appropriate repair mechanism. For example, ionizing radiation that can cause double-stranded breaks in DNA activates ATM protein that sets in motion a chain of molecular interactions that involve repair mechanisms such as Non-homologous End Joining, Homologous Repair, and Nucleotide Excision Repair pathway. Kinases like ATM and ATR respond to replication blocks in two distinct processes that operate on different timescales: (i) relatively fast post-translational modifications like phosphorylation of downstream kinases ultimately leading to the inhibition of the cell cycle phosphatase CDC25 required for CDK activation (ii) slower transcriptional regulations, the most well-studied of which, is the role of p53.

P53 is a transcription factor that can regulate the expression of proteins that play critical roles in cell cycle arrest, apoptosis, or senescence. In healthy cells, p53 is maintained in low concentrations. Upon detecting double-strand breaks, ATM activates p53 by phosphorylation. This results in the expression of the CDK inhibitor p21 and the pro-apoptotic BAX and PUMA proteins. p21 arrests cell cycle by inhibiting cyclin–CDK complexes that phosphorylate proteins mediating G1 to S phase transition. Hence, p53 is critical to the G1/S checkpoint mechanism. In cells where p53 is mutated or absent, cell division can no longer be regulated, and such an uncontrolled cell division results in malignant tumors. Additionally, p53 can directly activate repair pathways such as NER via the regulation of factors that mediate Nucleotide Excision Repair and induce dNTP synthesis.


Suggested Reading

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter