19.4: Méiose vs Mitose
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is prevented during mitosis and meiosis I by high cyclin-dependent kinase (CDK) activity. This activity stops the formation of pre-replicative complexes and thus does not allow DNA replication to begin. No DNA synthesis occurs before the initiation of meiosis II as CDK activity remains high in both meiosis I and II.
Meiosis and mitosis also employ different damage repair pathways. Accidental DNA damage can result in double-strand breaks (DSBs) in DNA, which can be repaired by either non-homologous DNA end-joining or homologous recombination. In mitosis, accidental and random DSBs are repaired by the homologous recombination repair process. In contrast, pairing and recombination of the homologous chromosomes are a part of meiosis I, and therefore DSBs formation and repair occur in each meiosis cycle. Unlike mitosis, these DSBs are formed at selected places on the chromosome by the endonuclease Spo11.
Errors can occur during chromosome segregation resulting in the production of aneuploid cells. Female meiosis is more error-prone than mitosis and male meiosis. Though the exact reasons for a high error rate in female meiosis still need to be determined, several factors are considered responsible. The single meiosis cycle in an oocyte can take up to 40 years to complete, ending only after fertilization. Such a long duration can cause cohesin deterioration resulting in the premature release of sister chromatids. The chiasmata holding two homologous chromosomes can also slip away with time, affecting the correct orientation and attachment of the bivalents to the meiotic spindle. Also, aging can cause a decrease in the concentration of proteins (e.g., Mad2) involved in the spindle-assembly checkpoint mechanism, which can be another reason for the relatively high error rate in female meiosis.
The missegregation of chromosomes can cause the development of zygotes into abnormal embryos, which often die during fetal development or just after birth. It can also cause genetic diseases like Down syndrome and Turner syndrome. The chances of chromosomal segregation errors have been found to increase with the age of the mother. For example, a woman below 30 years of age has less than a 0.1% chance to have a baby with Down syndrome, whereas the chance increases to 3.5% for a woman who is 45 years old.
