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35.13: Anaphase Promoting Complex
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Cell Biology

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Anaphase Promoting Complex
 
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35.13: Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and activates it, resulting in the formation of  APCCdc20 and APCCdh1 complexes, respectively. Cdc20 and Cdh1 also help APC to identify its substrates.

APCCdc20 plays an essential role in metaphase to anaphase transition. The sister chromatids are held together by cohesins. The cohesin rings need to be broken by the enzyme separase so that the sister chromatids can move towards the opposite poles during anaphase. However, separase activity is inhibited by the protein, securin. APCCdc20 ubiquitinates securin, leading to its proteasomal degradation. This leads to the subsequent activation of the separase. APCCdc20 also ubiquitinates mitotic cyclins, which in turn inactivates cyclin-dependent kinases or Cdks. The inactivation of Cdks and the subsequent dephosphorylation of Cdk-substrates are necessary for the completion of cell division.


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Anaphase Promoting Complex Also Known As APC Is A Crucial Protein Complex Involved In The Regulation Of Cell Division. It Plays A Key Role In Controlling The Progression Of Cells From Metaphase To Anaphase During Mitosis And From G1 To S Phase During The Cell Cycle. The Main Function Of The Anaphase Promoting Complex Is To Mark Specific Proteins For Degradation By Attaching Chains Of Ubiquitin Molecules To Them. This Ubiquitination Process Targets These Proteins For Destruction By The Proteasome Ensuring That They Are Removed From The Cell At The Appropriate Time. APC Is Composed Of Multiple Subunits That Work Together To Carry Out Its Functions. These Subunits Include Core Components Such As APC2 APC11 And APC3 As Well As Regulatory Subunits That Help To Control Its Activity. The Activity Of APC Is Tightly Regulated At Different Stages Of The Cell Cycle To Ensure Proper Timing And Coordination Of Cell Division Processes. Dysregulation Or Mutations In The Anaphase Promoting Co

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