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A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk

1Department of Surgery, University of Michigan Medical School, 2Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, 3Graduate Program in Immunology, University of Michigan Medical School, 4Graduate Program in Cellular and Molecular Biology, University of Michigan Medical School, 5Undergraduate Research Opportunity Program, University of Michigan, 6Department of Surgery, Ann Arbor Veterans Affairs Healthcare System

JoVE 60486

 Bioengineering

Optocardiography and Electrophysiology Studies of Ex Vivo Langendorff-perfused Hearts

1Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Hospital, 2Children's National Heart Institute, Children's National Hospital, 3Center for Neuroscience Research, Children's National Hospital, 4Department of Biomedical Engineering, School of Engineering, University of Alabama at Birmingham, 5Department of Pediatrics, Department of Pharmacology & Physiology, School of Medicine and Health Sciences, George Washington University

JoVE 60472

 Medicine

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

1Unit of Microscopy and Dynamic Imaging, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain, 2Centro di Imaging Sperimentale, Ospedale San Raffaele, Milan, Italy, 3Unit of Gynecological Oncology Research, European Institute of Oncology IRCCS, Milan, Italy

JoVE 60398

 Biology

Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

JoVE 59678

 Bioengineering
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