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Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

JoVE 55762


 Biochemistry

Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response

1Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, 2Department of Dermatology, University of Utah Health Sciences Center, Salt Lake City

JoVE 55712


 Cancer Research

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

JoVE 54599


 Immunology and Infection

The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight

1School of Science, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 2BlueScope Steel Research, 3Infrared Microspectroscopy Beamline, Australian Synchrotron, 4School of Science, College of Science, Engineering and Health, RMIT University

JoVE 54309


 Engineering

Microfluidic Pneumatic Cages: A Novel Approach for In-chip Crystal Trapping, Manipulation and Controlled Chemical Treatment

1Empa - Swiss Federal Laboratories for Materials Science and Technology, 2Institute of Chemical and Bioengineering, Department of Chemistry and Applied Bioscience, ETH Zurich, 3ICN2-Institut Catala de Nanociencia i Nanotecnologia, 4WITec GmbH, 5Institut de Ciència de Materials de Barcelona, 6School of Chemistry, The University of Nottingham

JoVE 54193


 Chemistry

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara

JoVE 54102


 Bioengineering

Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation

1Anesthesia Center for Critical Care Research of the Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, 2Cardiovascular Research Center and Cardiology Division of the Department of Medicine, Massachusetts General Hospital, 3Cardiovascular Division, Brigham and Women's Hospital, 4Harvard Medical School, 5Department of Anesthesiology, Uniklinik RWTH Aachen, RWTH Aachen University, 6Center for Immunology and Inflammatory Diseases and the Division of Rheumatology, Allergy, and Immunology of the Department of Medicine, Massachusetts General Hospital

JoVE 54017


 Medicine

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