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Buffers - Student Protocol

JoVE 11154

Source: Smaa Koraym at Johns Hopkins University, MD, USA



Preparing 50 mM NaH2PO4 Buffer, pH 7

In the first part of this experiment, you will prepare a sodium phosphate solution buffered at pH 7.0. Monosodium phosphate is a weak acid with the conjugate base, disodium phosphate. Unadjusted monosodium phosphate…

 Lab: Chemistry

Acid and Base Concentrations - Student Protocol

JoVE 11151

Source: Smaa Koraym at Johns Hopkins University, MD, USA



Preparation of ~0.1 M NaOH

In the first part of the lab, you will use a 50% w/w solution of NaOH to prepare 500 mL of ~0.1 M. The 50% w/w NaOH is indicative of its weight ratio. For example, if the instructor prepared 150 mL of the 50% w/w NaOH solution, then 150 g of NaOH …

 Lab: Chemistry

Galvanic Cells - Student Protocol

JoVE 11178

Source: Smaa Koraym at Johns Hopkins University, MD, USA



Constructing a Lead-Copper Galvanic Cell

In this experiment, you will construct a lead-copper galvanic cell and then measure the voltage that is generated during the redox reactions occurring at the anode and cathode.




To begin, put on the…

 Lab: Chemistry

Enzyme Activity - Student Protocol

JoVE 10586

Baseline Peroxidase Enzyme Experiment
To begin, the baseline for the peroxidase enzyme reaction must be determined. Make the substrate by adding 7 mL of distilled water to a clean test tube and then add 0.2 mL of guaiacol. Note: Guaiacol is a color-changing indicator that becomes more yellow-orange as the enzyme reaction progresses.
Next, add 0.3…

 Lab Bio

Photosynthesis - Student Protocol

JoVE 10566

Plant Pigment Chromatography
NOTE: In this experiment you will separate pigments from spinach leaves using chromatography paper. Individual pigments travel along the paper at different rates and may have different colors. By calculating the relative distance the pigments travel, their resolution factor, and comparing them with literature values, you can …

 Lab Bio

Hardy-Weinberg & Genetic Drift - Student Protocol

JoVE 10560

Class Simulation
Begin by opening a new spreadsheet file. Following the Hardy-Weinberg equation where p is the frequency of a dominant allele A in a population, and q is defined as the frequency of a recessive allele B, input frequency p of allele A into cell B2, and frequency q of allele B into cell B3.
Assign the value 0.5 to cell C2.
Follo…

 Lab Bio

UV-Vis Spectroscopy of Dyes - Prep Student

JoVE 11224

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Preparation of Dye Solutions

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




First, put on a lab coat, safety glasses, and nitrile…

 Lab: Chemistry
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