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Climate Change - Student Protocol

JoVE 10610

Melting Land and Sea Ice
NOTE: This activity is a simulation of land- and sea-ice melting and their effects on water levels.
First, label one graduated cylinder as 'Sea Ice' and the other graduated cylinder as 'Land Ice.' Hypotheses: the experimental hypothesis might be that the land ice melting will cause the water level to rise more than the…

 Lab Bio

Climate Change- Concept

JoVE 10609

The certainty of climate change remains a public controversy despite the consensus among approximately 97% of active climate researchers, who not only agree that the Earth’s climate is changing but also state that this change is intensified by human activity, predominantly carbon emissions 1. The disconnect between the public and the experts is partly due to poor understanding of the…

 Lab Bio

Community Diversity - Student Protocol

JoVE 10608

Measuring Biodiversity in Areas with Different Disturbance Regimes
NOTE: In this experiment, you will compare the biodiversity in a less disturbed area against a more highly disturbed area. HYPOTHESES: The experimental hypothesis could be that a less disturbed area will contain a greater diversity of species than a highly disturbed area. The null…

 Lab Bio

Population Growth - Student Protocol

JoVE 10606

Exponential Population Growth Model
NOTE: In these experiments you will use computer software to simulate different types of growth models. HYPOTHESES: The experimental hypothesis might be that if we increase either R, the reproductive rate or the initial population size, NT, that this will increase the population size after 10 generations in an…

 Lab Bio

Animal Behavior - Student Protocol

JoVE 10583

Making Choice Chambers
Before beginning the experiments, use one of the Sharpies from the lab table to trace a line around the circumference of the bottom of both bottles. One line should be 1 cm from the base of the bottle and the other should be 1.5 cm from the base of the bottle.
Use a razor blade to make a small cut in each bottle at the mark. …

 Lab Bio

Microscopy and Staining: Gram, Capsule, and Endospore Staining

JoVE 10513

Source: Rhiannon M. LeVeque1, Natalia Martin1, Andrew J. Van Alst1, and Victor J. DiRita1
1 Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, United States of America


Bacteria are diverse microorganisms found nearly everywhere on Earth. Many properties help distinguish them from…

 Microbiology

MicroRNAs

JoVE 10801

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends have been methylated to prevent degradation, it is exported from the nucleus into the cytoplasm. In the cytoplasm, another endonuclease enzyme, called Dicer, cuts the pre-miRNA into a 21-24 nucleotide-long miRNA duplex. Then, Dicer cleaves one strand of the duplex, releasing a single strand of mature miRNA. The mature miRNA is loaded into a protein complex called RNA-induced silencing complex (RISC), which the miRNA then guides to the complementary region of its target mRNA. The extent of complementary base-pairing between miRNA and 3’ untranslated region of target mRNA determines the gene silencing mechanism. Extensive or near-perfect complementarity causes degradation of mRNA, whereas limited base-pairing inhibits translation. While silencing via mRNA degradation is irreversible, translation inhibition is reversible since stable mRNA can

 Core: Biology

Microbial and Fungal Diversity - Prep Student

JoVE 10638

Nutrient Agar and Bacterial Culture Plate Preparation
Add 23 g of nutrient agar to a 2,000 mL beaker containing 1 L of distilled water 4 – 5 days before the activity.
Place the beaker on a hotplate and set the hotplate to high, stirring the mixture with a stirring rod.
When the agar has dissolved into the water, use a hot pad to…

 Lab Bio

Measuring Biodiversity - Student Protocol

JoVE 10635

Quadrats and Transects
In this activity, you will be using quadrats along transect lines to take biodiversity measurements of the plant species in three distinct habitats near your classroom. Begin by splitting into groups. Four works best.
Select one person in your group to be the data recorder. The other three will be the data collectors.
N…

 Lab Bio

Enzyme Activity - Prep Student

JoVE 10584

Investigating the Effect of pH and Temperature on Peroxidase Activity
To make the extraction buffer for the peroxidase enzyme, mix equal volumes of 0.1 M sodium phosphate monobasic and 0.1 molar sodium phosphate dibasic solutions to achieve a final volume of 500 mL.
Test the resulting solution using a pH meter. The mixture should be between a pH…

 Lab Bio
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