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October, 2006
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Photosynthesis - Prep Student

JoVE 10623

Plant Pigment Chromatography and Floating Leaf Discs in a Vacuum
On the day before the experiment, fill a container with water.
Place the spinach leaves into the water and make sure they are submerged.
Leave the spinach leaves to soak overnight at room temperature.
Wearing gloves, prepare and measure out 18 cm x 304 cm sections of…

 Lab Bio

Hardy-Weinberg & Genetic Drift - Prep Student

JoVE 10620

Mathematical Modeling

Before beginning the mathematical modeling exercise make sure each computer has access to an either on or offline spreadsheet software program.

Simulation for Hardy-Weinberg and Genetic Drift

Prepare one set of beads…

 Lab Bio

Macromolecules - Prep Student

JoVE 10589

Solution Preparation
CAUTION: It is important to always use care when handling reagents. For example, iodine will stain clothes and skin so wear gloves, goggles, and a lab coat when using this reagent.
To prepare a 1 M glucose solution, begin by using a stir bar to dissolve 180.2 g of glucose in 500 mL of distilled water, increasing the volume to…

 Lab Bio

16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species

JoVE 10510

Source: Ewa Bukowska-Faniband1, Tilde Andersson1, Rolf Lood1
1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden

Planet Earth is a habitat for millions of bacterial species, each of which has specific characteristics. Identification of bacterial species is…


An Overview of Alkenone Biomarker Analysis for Paleothermometry

JoVE 10219

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst

Throughout this series of videos, natural samples were extracted and purified in search of organic compounds, called biomarkers, that can relate information on climates and environments of the past. One of the samples analyzed was sediment. Sediments accumulate…

 Earth Science

Piaget's Conservation Task and the Influence of Task Demands

JoVE 10131

Source: Laboratories of Judith Danovitch and Nicholaus Noles—University of Louisville

Jean Piaget was a pioneer in the field of developmental psychology, and his theory of cognitive development is one of the most well-known psychological theories. At the heart of Piaget’s theory is the idea that children’s ways of…

 Developmental Psychology

Visualizing Soil Microorganisms via the Contact Slide Assay and Microscopy

JoVE 10053

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Bradley Schmitz

Soil comprises the thin layer at the earth’s surface, containing biotic and abiotic factors that contribute to life. The abiotic portion includes inorganic particles ranging in size and shape that determine…

 Environmental Microbiology

Dihybrid Crosses

JoVE 10774

To determine whether traits are inherited together or separately, Gregor Mendel crossed pea plants that differed in two traits. These parental plants were homozygous for both traits but displayed different phenotypes. The first generation of offspring were all dihybrids, heterozygotes exhibiting the two dominant phenotypes. When self-fertilized, the dihybrids consistently produced progeny with a 9:3:3:1 ratio of four possible phenotype combinations. This ratio suggested that inheriting one trait did not affect the likelihood of inheriting the other, establishing Mendel’s law of independent assortment. Gregor Mendel’s monohybrid crosses, between pea plants that differed in a single trait, demonstrated that (1) organisms randomly inherit one of two copies of each gene from each parent (Mendel’s first law, segregation), and (2) the dominant allele can mask the recessive allele’s effects on phenotype (the principle of uniformity). To determine whether two traits were inherited separately or together, Mendel also performed crosses with pea plants that differed in two traits, such as pea color and pea shape. For these dihybrid crosses, Mendel first mated plants that were true breeding (i.e., homozygous) for different traits of the same two characteristics. For example, he crossed plants that bred true for round, yellow peas (RRYY

 Core: Biology

Eusociality and Division of Labor - Prep Student

JoVE 10646

Simulating Eusocial and Solitary Behavior
Choose an open location for the activity to take place, with the center area clear of any items that students may trip over.
Set out four 1-gallon buckets and add 5 pounds of rice to each one.
Next, add ¼ - ½ cup of dried black beans to the first bucket and mix the beans into the rice.…

 Lab Bio

Sexual Selection and Mate Choice - Prep Student

JoVE 10645

Determining Roles and Making Gamete Packages for Class
First determine the quantity of materials needed based on the class size. This activity can easily be scaled up or down. In Table 1, options are listed for 30, 20 or 10 students. Click Here to download Table 1 NOTE: You may easily create a custom number by…

 Lab Bio
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