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October, 2006
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Enzyme Activity- Concept

JoVE 10585

Biological Catalysts

All living organisms continuously perform numerous biochemical reactions to sustain their presence. Most of these reactions require an input of energy to start, which is called the activation energy. Catalysts are chemicals that lower the activation energy. Even though catalysts facilitate a chemical reaction, they are not consumed by it. This means a catalyst …

 Lab Bio

Transpiration- Concept

JoVE 10580


All living organisms must carry out a set of basic functions in order to maintain themselves. One of these processes involves the transportation of materials throughout the organism. Therefore, organisms need to exchange materials with the environment, which can be seen on the small scale of cells transporting protein and other materials among one another or on a larger …

 Lab Bio

DNA Isolation and Restriction Enzyme Analysis - Prep Student

JoVE 10575

Preparation of Solutions and Materials
NOTE: For this lab to run smoothly, it is recommended to not exceed 12 samples per laboratory class.
First gather the necessary materials, including popsicle sticks, funnels, 15 mL conical tubes, micropipettes, ice buckets with ice, small cups and microcentrifuge tubes.
Next set the water bath used in…

 Lab Bio

Bacterial Transformation- Concept

JoVE 10573


In early 20th century, pneumonia was accountable for a large portion of infectious disease deaths1. In order to develop an effective vaccine against pneumonia, Frederick Griffith set out to study two different strains of the Streptococcus pneumoniae: a non-virulent strain with a rough appearance (R-strain) and a virulent strain with a smooth appearance…

 Lab Bio

Cell Division - Student Protocol

JoVE 10572

Observing the Cell Cycle in a Root Tip
Hypotheses: The experimental hypothesis is that in root tips slices that have been treated with nocodazole, a chemical that interferes with microtubular polymerization, all of the cells will be arrested at the same stage of the cell cycle and that in untreated onion tip slices all of the different stages of the…

 Lab Bio

Physiology of the Circulatory System - Prep Student

JoVE 10569

Measuring Blood Pressure
To prepare for the blood pressure exercise, simply place the appropriate number of alcohol swabs, sphygmomanometers, and stethoscopes at the front of the classroom.
Be sure to check over each of the component parts of the sphygmomanometers, including the tubing, cuff, manometer, and bulb to ensure they are undamaged.…

 Lab Bio

Cellular Respiration - Student Protocol

JoVE 10568

Quantifying Respiration using Microrespirometers
NOTE: In this experiment, you will measure the rate of cellular respiration for germinating seeds by measuring the rate of exchange for oxygen. As oxygen is consumed to provide energy, germinating seeds release carbon dioxide. This carbon dioxide is absorbed by potassium carbonate and thus the overall…

 Lab Bio

Cellular Respiration- Concept

JoVE 10567

Autotrophs and Heterotrophs

Living organisms require a continuous input of energy to maintain cellular and organismal functions such as growth, repair, movement, defense, and reproduction. Cells can only use chemical energy to fuel their functions, therefore they need to harvest energy from chemical bonds of biomolecules, such as sugars and lipids. Autotrophic organisms, namely…

 Lab Bio

Diffusion and Osmosis - Prep Student

JoVE 10563

Preparation of Solutions for the Agar Cube Experiment
IMPORTANT: Wear gloves, goggles, and appropriate personal protective equipment – chemicals can be hazardous at high concentrations.
For the diffusion indicator solution, weigh out 1 g of phenolphthalein and add it to a beaker containing 100 mL of 95% ethanol.
To make the basic…

 Lab Bio

Artificial Selection - Student Protocol

JoVE 10556

Observing Artificial Selection in Plants
To begin the experiment, collect five two-week old grown plants from the generation one population that appear healthy. Note: Plants should appear green, not wilted, and not have damaged leaves. Experimental Hypothesis: The experimental hypothesis for this lab might be that the number of lead trichomes varies…

 Lab Bio
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