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CRISPR/Cas9-Mediated Targeted Integration In Vivo Using a Homology-Mediated End Joining-Based Strategy

1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 2College of Life Sciences, University of Chinese Academy of Sciences, 3School of Life Science and Technology, Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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JoVE 56844


 JoVE In-Press

Intracavernosal Pressure Recording to Evaluate Erectile Function in Rodents

1State Key Laboratory of Reproductive Medicine, Department of Andrology, Maternity Hospital Affiliated to Nanjing Medical University, 2School of Pharmaceutical Sciences, Nanjing Tech University, 3State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, Nanjing University, 4Department of Urology, Affiliated Drum Tower Hospital, School of Medicine, Nanjing University, 5Department of Ultrasound, Nanjing Qixia District Maternity and Child Health Care Hospital

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JoVE 56798


 JoVE In-Press

Dissecting Multi-Protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

1The Kinghorn Cancer Centre, Garvan Institute of Medical Research, 2Ubiquitin Signaling Group, Protein Signaling Program, The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3RCSI Molecular Medicine, Royal College of Surgeons in Ireland, 4Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, 5School of Medical Sciences, University of New South Wales, 6St Vincent's Hospital Clinical School, University of New South Wales, 7School of Medicine and Medical Science, University College Dublin

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JoVE 57109


 JoVE In-Press

Technique and Patient Selection Criteria of Right Anterior Mini-Thoracotomy for Minimal Access Aortic Valve Replacement

1Department of Cardiovascular and Thoracic Surgery, Pitié Salpêtrière University Hospital, Assistance Publique, Hôpitaux de Paris (APHP), Institut de Cardiologie, 2Institute of Veterinary Physiology and Zurich Center for Integrative Human Physiology, University of Zurich, 3Herzzentrum Hirslanden, Klinik St Anna, 4Department of Radiology, Canton Hospital Lucerne, 5Department of Anesthesiology and Intensive Care Unit, Pitié Salpêtrière University Hospital, Assistance Publique, Hôpitaux de Paris (APHP), Institut de Cardiologie

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JoVE 57323


 JoVE In-Press

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-Canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

1Institute of Chemistry L 1, Department of Biocatalysis, Technical University of Berlin, 2Institute of Chemistry PC 14, Department of Bioenergetics, Technical University of Berlin, 3Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences, Technical University of Berlin

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JoVE 57017


 JoVE In-Press

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

1The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 3Trauma Office, Children's National Health System

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JoVE 56761


 JoVE In-Press

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