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Bacteria: One of the three domains of life (the others being Eukarya and Archaea), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to Oxygen: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or Phototrophy (via light reaction); for chemotrophs by their source of chemical energy: Chemolithotrophy (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for Carbon; Nitrogen; etc.; Heterotrophy (from organic sources) or Autotrophy (from Carbon dioxide). They can also be classified by whether or not they stain (based on the structure of their Cell walls) with Crystal violet dye: gram-negative or gram-positive.

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

1Focal Area Infection Biology, Biozentrum, University of Basel, 2Centre d’Immunologie de Marseille-Luminy, Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical, Universidad de Navarra, 4BioDataAnalysis GmbH

JoVE 54263


 Immunology and Infection

A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

1Department of Biochemistry, Stanford University School of Medicine, 2Department of Mechanical and Aerospace Engineering, University of California San Diego, 3Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute, Stanford University School of Medicine

JoVE 57361


 Immunology and Infection

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