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Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

In Vitro Microfluidic Disease Model to Study Whole Blood-Endothelial Interactions and Blood Clot Dynamics in Real-Time

1Department of Pulmonary Diseases, Amsterdam UMC, VU University Medical Center, Amsterdam Cardiovascular Sciences (ACS), 2BIOS Lab-on-a-Chip group, University of Twente, 3Applied Stem Cell Technologies Group, University of Twente, 4Department of Cardio-thoracic Surgery, Amsterdam UMC, VU University Medical Center


JoVE 61068

 JoVE In-Press

A Xenograft Mouse Model to Assess Efficacy of Therapeutic Agents for Human Acute Leukemia

1Department of Pediatrics, Pennsylvania State University College of Medicine, 2Penn State Hershey Cancer Institute, Pennsylvania State University College of Medicine, 3Departments of Medicine, Division of Hematology and Oncology, Pennsylvania State University College of Medicine, 4University of Virginia Cancer Center, 5nanoSTAR Institute, University of Virginia, 6Division of Hematology and Oncology, Department of Medicine, University of Virginia School of Medicine, 7Department of Pharmacology, Pennsylvania State University College of Medicine


JoVE 60980

 JoVE In-Press

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

1CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 2School of Life Science, University of Science and Technology of China, 3State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences


JoVE 60523

 JoVE In-Press

Flux-Based Assay for the Identification of Autophagy Modulators for Osteoarthritis

1Grupo de Biología del Cartílago, Servicio de Reumatología, Instituto de Investigación Biomédica de A Coruña (INIBIC), Complejo Hospitalario Universitario de A Coruña, 2Biofarma Research Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidad de Santiago de Compostela


JoVE 61078

 JoVE In-Press
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