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Lasers: An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (Laser) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.

Förster Resonance Energy Transfer (FRET)

JoVE 5696

Förster resonance energy transfer (FRET) is a phenomenon used to investigate close-range biochemical interactions. In FRET, a donor photoluminescent molecule can non-radiatively transfer energy to an acceptor molecule if their respective emission and absorbance spectra overlap. The amount of energy transferred—and consequently the overall emission of sample—depends on the…

 Biochemistry

Analysis of Thermal Expansion via Dilatometry

JoVE 10490

Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT


Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a…

 Materials Engineering

Electric Fields

JoVE 10322

Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN


An electric field is generated by a charged object (referred to as the source charge) in the space around it, and represents the ability to exert electric force on another charged object (referred to as the test…

 Physics II

Raman Spectroscopy for Chemical Analysis

JoVE 5701

Source: Laboratory of Dr. Ryoichi Ishihara — Delft University of Technology


Raman spectroscopy is a technique for analyzing vibrational and other low frequency modes in a system. In chemistry it is used to identify molecules by their Raman fingerprint. In solid-state physics it is used to characterize materials, and more specifically …

 Analytical Chemistry

Live Cell Imaging of Mitosis

JoVE 5642

Mitosis is a form of cell division in which a cell’s genetic material is divided equally between two daughter cells. Mitosis can be broken down into six phases, during each of which the cell’s components, such as its chromosomes, show visually distinct characteristics. Advances in fluorescence live cell imaging have allowed scientists to study this process in…

 Cell Biology
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