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Buffers - Student Protocol

JoVE 11154

Preparing 50 mM NaH2PO4 Buffer, pH 7

In the first part of this experiment, you will prepare a sodium phosphate solution buffered at pH 7.0. Monosodium phosphate is a weak acid with the conjugate base, disodium phosphate. Unadjusted monosodium phosphate solutions usually have a pH of about 4 - 6.


Buffers are most…

 Lab: Chemistry

UV-Vis Spectroscopy - Prep Student

JoVE 11224

Preparation of Dye Solutions

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




First, put on a lab coat, safety glasses, and nitrile gloves. Some solutions use hexane or dimethylformamide, so prepare those solutions in a fume hood.…

 Lab: Chemistry

Steam Distillation- Concept

JoVE 11204

Steam Distillation

Steam distillation is a separation technique that harnesses the low boiling point property of immiscible mixtures. It is predominately used to separate temperature-sensitive organic molecules from a non-volatile contaminant. The organic molecule must be immiscible in water.


In steam distillation, the immiscible mixture is heated to…

 Lab: Chemistry

Beer's Law - Student Protocol

JoVE 11169

Lab Setup for Beer’s Law

In this lab, you will combine aqueous solutions of FeCl3 and NaSCN to form an orange-red [Fe(NCS)]2+ complex. When FeCl3 is dissolved in water, the iron center is surrounded by six water molecules. This acidic complex easily loses protons from its water molecules, which can lead to iron hydroxides precipitating from…

 Lab: Chemistry

Extraction - Prep Student

JoVE 11197

Preparation of the Laboratory

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




Put on a lab coat, splash-proof safety glasses, and nitrile gloves.


Place labeled containers for halogenated waste and solid…

 Lab: Chemistry

Beer's Law - Prep Student

JoVE 11167

Preparation of Solutions

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. Note: …

 Lab: Chemistry

Acid and Base Concentrations- Concept

JoVE 11150

Acids and Bases

An Arrhenius acid produces hydrogen ions when it is dissolved in water:


HA + H2O → H+(aq) + A-(aq)


and an Arrhenius base produces hydroxide ions when dissolved in water:


B + H2O → BH+(aq) + OH-(aq)


Here, HA is the non-dissociated acid and H+ is the…

 Lab: Chemistry

Transpiration - Student Protocol

JoVE 10581

Comparing Transpiration Rates of Leaves
To begin the experiment to measure the transpiration rates of leaves from different plant species, you will first create a tool called a potometer, using a piece of plastic tubing and 5 mL calibrated pipette.
To assemble the device, simply attach the rubber tubing to the tip of the pipette.
Then,…

 Lab Bio

Concentration Dependence - Prep Student

JoVE 11170

Preparation of Solutions

Here, we show the preparation of solutions for 10 students working in pairs, with some excess. Please adjust quantities as needed.




Put on a lab coat, splash-proof safety glasses, and nitrile gloves. Obtain a waste container for aqueous sodium thiosulfate in advance. Note:…

 Lab: Chemistry

Stoichiometry, Product Yield, and Limiting Reactants - Prep Student

JoVE 11140

Preparation of 25% v/v Ethylenediamine in Deionized Water

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash…

 Lab: Chemistry
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