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Paraffin: A mixture of solid hydrocarbons obtained from petroleum. It has a wide range of uses including as a stiffening agent in ointments, as a lubricant, and as a topical anti-inflammatory. It is also commonly used as an embedding material in histology.

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

JoVE 57471


Concentration Dependence - Student Protocol

JoVE 11172

Concentration Dependence

In this experiment, you will combine varying concentrations of aqueous HCl and sodium thiosulfate to make solid sulfur, which rapidly gathers into opaque yellow particles at a certain sulfur concentration. Since the reaction solution starts clear and colorless, you can easily tell when it reaches that concentration.

 Lab: Chemistry

Buffers - Prep Student

JoVE 11152

Preparation of Solutions

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.

To set up for this lab experiment, wear the appropriate personal protective equipment, including a lab coat, chemical splash goggles, and gloves. All solutions …

 Lab: Chemistry

Synthesis of Luminol - Student Protocol

JoVE 11217

Synthesis of 3-Nitrophthalhydrazide

In this lab, you'll synthesize 3-aminophthalhydrazide, which is also called luminol, in a 2-step process. The first step is a condensation reaction between 3-nitrophthalic acid and hydrazine.

During this step, the carboxylic acid groups are substituted with NH groups to produce…

 Lab: Chemistry

Galvanic Cells - Student Protocol

JoVE 11178

Constructing a Lead-Copper Galvanic Cell

In this experiment, you will construct a lead-copper galvanic cell and then measure the voltage that is generated during the redox reactions occurring at the anode and cathode.

To begin, put on the necessary personal protective equipment, including a lab coat, gloves, and chemical …

 Lab: Chemistry

Beer's Law - Student Protocol

JoVE 11169

Lab Setup for Beer’s Law

In this lab, you will combine aqueous solutions of FeCl3 and NaSCN to form an orange-red [Fe(NCS)]2+ complex. When FeCl3 is dissolved in water, the iron center is surrounded by six water molecules. This acidic complex easily loses protons from its water molecules, which can lead to iron hydroxides precipitating from…

 Lab: Chemistry

Histological Sample Preparation for Light Microscopy

JoVE 5039

Histology is the study of cells and tissues, which is typically aided by the use of a light microscope. The preparation of histological samples can vary greatly based on the inherent properties of the samples such as size and hardness as well as expected post-processing which includes planned staining techniques or other down-stream applications. As described in this video, specimen…

 General Laboratory Techniques
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