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Scientific Measurement and Lab Skills - Student Protocol

JoVE 11139

Source: Smaa Koraym at Johns Hopkins University, MD, USA



Measuring Diameter and Circumference

Error can be introduced in any experiment, even when the technique is performed carefully and correctly. It is essential to critically examine your experimental methods when performing any lab exercise in order to understand and…

 Lab: Chemistry

Transpiration - Student Protocol

JoVE 10581

Comparing Transpiration Rates of Leaves
To begin the experiment to measure the transpiration rates of leaves from different plant species, you will first create a tool called a potometer, using a piece of plastic tubing and 5 mL calibrated pipette.
To assemble the device, simply attach the rubber tubing to the tip of the pipette.
Then,…

 Lab Bio

Esterification - Student Protocol

JoVE 11220

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Esterification

In this lab, you will synthesize an ester from a carboxylic acid and an alcohol in the presence of sulfuric acid. This reaction is called Fischer esterification. The sulfuric acid makes the carboxylic acid more reactive towards the alcohol. …

 Lab: Chemistry

Basic Organic Chemistry Techniques - Student Protocol

JoVE 11187

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Synthesis of Benzoic Acid

In this lab, you'll learn several important techniques that you'll use throughout this class. This includes measuring the mass or volume of reactants and products, methods of heating and cooling reaction mixtures, proper…

 Lab: Chemistry

Proper Lab Notebook Keeping - Student Protocol

JoVE 11184

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Nitration of Methyl Benzoate

From this lab onward, you'll keep a record of every experiment in your lab notebook. This starts with preparing your lab notebook before you come to the lab. This preparation, which is often called a pre-lab, typically…

 Lab: Chemistry

Beer's Law - Student Protocol

JoVE 11169

Source: Smaa Koraym at Johns Hopkins University, MD, USA



Lab Setup for Beer’s Law

In this lab, you will combine aqueous solutions of FeCl3 and NaSCN to form an orange-red [Fe(NCS)]2+ complex. When FeCl3 is dissolved in water, the iron center is surrounded by six water molecules. This acidic complex easily loses protons…

 Lab: Chemistry

Identifying Alcohols - Prep Student

JoVE 11227

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Preparation of Reagents

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




Before you begin, put on a lab coat, safety glasses, and nitrile …

 Lab: Chemistry

UV-Vis Spectroscopy of Dyes - Student Protocol

JoVE 11226

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Absorbance Wavelength Comparison

In the first part of the lab, you'll use ultraviolet and visible light absorption spectroscopy, or UV-Vis absorption spectroscopy, to analyze the absorption characteristics of fluorescein, β-carotene, and indigo…

 Lab: Chemistry

Column Chromatography - Student Protocol

JoVE 11211

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Column Chromatography of Food Dye

In this experiment, you'll use column chromatography to separate green food coloring into its component blue and yellow dyes, called erioglaucine and tartrazine. First, you'll use 95% ethanol to elute the dye with …

 Lab: Chemistry

Solubility - Student Protocol

JoVE 11160

Source: Smaa Koraym at Johns Hopkins University, MD, USA



Creating a Saturated Solution

In this experiment, you will create a saturated solution of sodium tetraborate decahydrate, also called borax. In water, borax dissociates into two sodium cations and one tetraborate anion. When an aqueous borax solution is saturated, it means …

 Lab: Chemistry
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