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Molecular Orbital (MO) Theory

JoVE 10447

Source: Tamara M. Powers, Department of Chemistry, Texas A&M University


This protocol serves as a guide in the synthesis of two metal complexes featuring the ligand 1,1'-bis(diphenylphosphino)ferrocene (dppf): M(dppf)Cl2, where M = Ni or Pd. While both of these transition metal complexes are 4-coordinate, they exhibit different…

 Inorganic Chemistry

ELISA Assays: Indirect, Sandwich, and Competitive

JoVE 10496

Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4
1 Department of Urology, University of Minnesota, Minneapolis, MN 55455
2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455
3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455
4 Masonic…

 Immunology

Nucleophilic Substitution

JoVE 10465

Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA


Nucleophilic substitution reactions are among the most fundamental topics covered in organic chemistry. A nucleophilic substitution reaction is one where a nucleophile (electron-rich Lewis base) replaces a leaving group from a carbon…

 Organic Chemistry II

Population Growth - Student Protocol

JoVE 10606

Exponential Population Growth Model
NOTE: In these experiments you will use computer software to simulate different types of growth models. HYPOTHESES: The experimental hypothesis might be that if we increase either R, the reproductive rate or the initial population size, NT, that this will increase the population size after 10 generations in an…

 Lab Bio

Basic Organic Chemistry Techniques - Prep Student

JoVE 11185

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Preparation of the Laboratory

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




To set up for this lab experiment, wear the appropriate…

 Lab: Chemistry

Eusociality and Division of Labor - Prep Student

JoVE 10646

Simulating Eusocial and Solitary Behavior
Choose an open location for the activity to take place, with the center area clear of any items that students may trip over.
Set out four 1-gallon buckets and add 5 pounds of rice to each one.
Next, add ¼ - ½ cup of dried black beans to the first bucket and mix the beans into the rice.…

 Lab Bio

Transformation of E. coli Cells Using an Adapted Calcium Chloride Procedure

JoVE 10515

Source: Natalia Martin1, Andrew J. Van Alst1, Rhiannon M. LeVeque1, and Victor J. DiRita1
1 Department of Microbiology and Molecular Genetics, Michigan State University


Bacteria have the ability to exchange genetic material (DeoxyriboNucleic Acid, DNA) in a process known as horizontal gene transfer. Incorporating exogenous DNA…

 Microbiology

Serial Dilutions and Plating: Microbial Enumeration

JoVE 10507

Source: Jonathan F. Blaize1, Elizabeth Suter1, and Christopher P. Corbo1
1 Department of Biological Sciences, Wagner College, 1 Campus Road, Staten Island NY, 10301


Quantitative assessment of prokaryotes can be onerous given their abundance, propensity for exponential proliferation, species diversity within a population, and specific …

 Microbiology
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