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Optical Materialography Part 2: Image Analysis

JoVE 10433

Source: Faisal Alamgir, School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA


The imaging of microscopic structures of solid materials, and the analysis of the structural components imaged, is known as materialography. Often, we would like to quantify the internal three-dimensional microstructure of a…

 Materials Engineering

Molecular Orbital (MO) Theory

JoVE 10447

Source: Tamara M. Powers, Department of Chemistry, Texas A&M University


This protocol serves as a guide in the synthesis of two metal complexes featuring the ligand 1,1'-bis(diphenylphosphino)ferrocene (dppf): M(dppf)Cl2, where M = Ni or Pd. While both of these transition metal complexes are 4-coordinate, they exhibit different…

 Inorganic Chemistry

Quadruply Metal-Metal Bonded Paddlewheels

JoVE 10441

Source: Corey Burns, Tamara M. Powers, Department of Chemistry, Texas A&M University


Paddlewheel complexes are a class of compounds comprised of two metal ions (1st, 2nd, or 3rd row transition metals) held in proximity by four bridging ligands (most commonly formamidinates or carboxylates) (Figure 1). Varying the…

 Inorganic Chemistry

Nucleophilic Substitution

JoVE 10465

Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA


Nucleophilic substitution reactions are among the most fundamental topics covered in organic chemistry. A nucleophilic substitution reaction is one where a nucleophile (electron-rich Lewis base) replaces a leaving group from a carbon…

 Organic Chemistry II

Population Growth - Student Protocol

JoVE 10606

Exponential Population Growth Model
NOTE: In these experiments you will use computer software to simulate different types of growth models. HYPOTHESES: The experimental hypothesis might be that if we increase either R, the reproductive rate or the initial population size, NT, that this will increase the population size after 10 generations in an…

 Lab Bio

Stoichiometry, Product Yield, and Limiting Reactants - Student Protocol

JoVE 11142

Source: Smaa Koraym at Johns Hopkins University, MD, USA



Synthesis of tris(ethylenediamine)nickel chloride

In this lab, you will combine nickel chloride hexahydrate and ethylenediamine in water to produce tris(ethylenediamine)nickel chloride. When nickel chloride hexahydrate dissolves in water, it rearranges to become the…

 Lab: Chemistry

ELISA Assays: Indirect, Sandwich, and Competitive

JoVE 10496

Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4
1 Department of Urology, University of Minnesota, Minneapolis, MN 55455
2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455
3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455
4 Masonic…

 Immunology

Determining the Solubility Rules of Ionic Compounds

JoVE 10197

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry


An ionic compound's solubility can be determined via qualitative analysis. Qualitative analysis is a branch of analytical chemistry that uses chemical properties and reactions to identify the cation or anion present in a chemical compound.…

 General Chemistry

Structure Of Ferrocene

JoVE 10347

Source: Tamara M. Powers, Department of Chemistry, Texas A&M University 


In 1951, Kealy and Pauson reported to Nature the synthesis of a new organometallic compound, ferrocene.1 In their original report, Pauson suggested a structure for ferrocene in which the iron is singly bonded (sigma bonds) to one carbon atom of…

 Inorganic Chemistry

Magnetic Activated Cell Sorting (MACS): Isolation of Thymic T Lymphocytes

JoVE 10495

Source: Meunier Sylvain1,2,3, Perchet Thibaut1,2,3, Sophie Novault4, Rachel Golub1,2,3
1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France
2 INSERM U1223, Paris, France
3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France
4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, …

 Immunology
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