Reagents: Recipes for many of the reagents used for these procedures are found in The Zebrafish Book, which is available in full online at http://zfin.org/zf_info/zfbook/zfbk.html. Recipes are provided there for preparation of Fish Water, Embryo Medium with and without antibiotics, pronase for dechorionating, fluorescent dextrans, and methyl cellulose.
Kemp, H. A., Carmany-Rampey, A., Moens, C. Generating Chimeric Zebrafish Embryos by Transplantation. J. Vis. Exp. (29), e1394, doi:10.3791/1394 (2009).