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Discussion
Drosophila 아이 imaginal 디스크의 해부는 간단하지만, 마스터 시간이 걸릴 수 있습니다. 중요한 단계는 눈 디스크와 멀리 떨어진 애벌레 두뇌로부터 절개의 식별됩니다. 아이 디스크의 식별은 눈 - RFP 라인이 여기에 설명된 사용하고 쉽게 구성되어 있습니다. 가능한 수정 antennal 디스크 또는 peripodial 멤브레인를 제거하거나 두뇌를 입력 홍보 axons을 시각화에 연결된 광 엽을 떠날 수 있습니다.
눈 - RFP 라인은 복제하여 pRed H - 스팅거 향상제 - 기자 벡터 3로지적 P2 유전자2 번째 인트론에서 소설 눈을 특정 향상제 요소를 생성하고 유전자 변형 파리를 만드는 이것을 사용했습니다. 눈 - RFP의 기자는 푸에르 토리코의 특정 하위 집합 및 원뿔 세포 (그림 2).로 표시됩니다. 그것은 따라서 관심의 유전자가 이러한 세포 유형에 표현인지 여부를 확인하는 데 사용하실 수 있습니다. 눈 - RFP 라인 요청하실 수 있습니다.
개발 Drosophila 아이는 PR neurogenesis 1 제어 유전자와 분자 경로를 공부위한 강력한 모델입니다. 여기에서 설명한 방법론과 기자는이 과정에 대한 이해를 더하기 위해 사용될 수 있습니다.
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Acknowledgments
이 작품은 킹스 칼리지 런던 왕립 학회에 의해 투자되었다.
References
Doroquez, D. B., Rebay, I. Crit Rev Biochem Mol Biol. 41 (6), 339-339 (2006).
Kaul, A. K., Craig, G. M., McNeill, H., Bateman, J. M. , Forthcoming.
Barolo, S., Castro, B., Posakony, J. W. Biotechniques. 36 (3), 436-436 (2004).
Kaul, A. K., Bateman, J. M. A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila. J. Vis. Exp. (34), e1617, doi:10.3791/1617 (2009).… More
Kaul, A. K., Bateman, J. M. A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila. J. Vis. Exp. (34), e1617, doi:10.3791/1617 (2009).
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