Southern Blotting para detectar recombinaciones homólogas en el genoma de células madre embrionarias de ratón
Published: May 31, 2023
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Abstract
Protocol
Disclosures
The authors have nothing to disclose.
Materials
| QIAquick Gel Extraction Kit | QIAGEN | 28704 | |
| DNA Denaturing Solution | VWR | 351-013-131 | |
| DNA Neutralizing Solution | VWR | 351-014-131 | |
| EcoR I | Thermo Scientific | ER0271 | |
| Dra I | Thermo Scientific | ER0221 | |
| ProbeQuan G-50 Micro Columns | GE Healthcare | 28-9034-08 | |
| Hybrisol I Hybridization Solution | Millipore | S4040 | |
| Ready-To-Go DNA Labeling Beads (-dCTP) | VWR | 27-9240-01 | |
| UltraPure SSC, 20x | Thermo Fisher | 15557036 | |
| Salmon Sperm DNA Solution | Thermo Fisher | 15632011 | |
| Whatman TurboBlotter Transfer System, Large Kits | Fisher Scientific | 09-301-188 | |
| [α32P] dCTP | PerkinElmer | NEG013H100UC | |
| Kodak X-Ray Film | Z&Z Medical | 844 5702 | |
| QIAprep Spin Miniprep Kit | QIAGEN | 27104 | |
| Nuclei Lysis Solution | Promega | A7941 | |
| Protein Precipitation Solution | Promega | A7951 |
Tags
Cite This Article
Southern Blotting to Detect Homologous Recombination in the Mouse Embryonic Stem Cell Genome. J. Vis. Exp. (Pending Publication), e21370, doi: (2025).