Materials
| Name | Company | Catalog Number | Comments |
| Micrococcal Nucleae | Sigma-Aldrich | N3755 | Make aliquots of 2 U/10μl buffer, stored at -20°C |
| Nunc MaxiSorp Microtiter Plates | Fisher Scientific | 12-565-135 | Adhesive plate covers can also be ordered through Fisher |
| Automated Plate Washer | |||
| 1-Step TMB-ELISAsubstrate | Pierce, Thermo Scientific | 34028 | Store at 4°C pre-warm to RT before use or as directed on the specification sheet |
| Microtiter Plate Reader | Bio-Rad | Most colorimetric plate reader models are suitable |
References
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- Jenuwein, T., Allis, C. D. Translating the histone code. Science. 293, 1074-1080 (2001).
- Turner, B. M. Histone acetylation and an epigenetic code. Bioessays. 22, 836-845 (2000).
- Dai, B., Rasmussen, T. P. Global epiproteomic signatures distinguish embryonic stem cells from differentiated cells. Stem Cells. 25, 2567-2574 (2007).
- Tanasijevic, B. Progressive accumulation of epigen etic heterogeneity during human ES cell culture. Epigenetics. 4, 330-338 (2009).
- Thomas, J. O. Isolation and fractionation of chromatin and linker histones. Chromatin: a practical approach. Gould, H. , Oxford University Press. Oxford. 12-14 (1998).
- Thorne, A. W., Cary, P. D., Crane-Robinson, C. Extraction and separation of core histones and non-histone chromosomal proteins. Chromatin: a practical approach. Gould, H. , Oxford University Press. Oxford. 38-39 (1998).
