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Neuroscience

وسحق العصب البصري النموذجي إصابة الفئران لدراسة الخلايا الشبكية بقاء العقدة

Published: April 25, 2011 doi: 10.3791/2685

Materials

Name Company Catalog Number Comments
Stereotaxic apparatus ASI Instruments SAS-4100
Dissecting microscope World Precision Instruments, Inc. PZMIV-BS
Scalpel handle Fine Science Tools 10003-12
Scalpel Blades #15 Fine Science Tools 10015-00
Microdrill Fine Science Tools 18000-17
Suture needle Fine Science Tools 12050-03
Spring scissors Fine Science Tools 16144-13
Dumont #5 forceps Fine Science Tools 11252-50
Hamilton syringe Hamilton Co 88400
Gauze sponges Office Depot 674889
Fluorochrome Sigma-Aldrich 39286
Alcohol Sigma-Aldrich 459844 70%
H2O2 Sigma-Aldrich H3410 3%

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References

  1. Yoles, E. GM1 reduces injury-induced metabolic deficits and degeneration in the rat optic nerve. Investigative ophthalmology & visual science. 33, 3586-3591 (1992).
  2. Fisher, J. Vaccination for neuroprotection in the mouse optic nerve: implications for optic neuropathies. J Neurosci. 21, 136-142 (2001).
  3. Levkovitch-Verbin, H. RGC death in mice after optic nerve crush injury: oxidative stress and neuroprotection. Investigative ophthalmology & visual science. 41, 4169-4174 (2000).
  4. Li, Y. VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats. The Journal of clinical investigation. 118, 913-923 (2008).
  5. Tang, Z. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. The Journal of experimental medicine. 207, 867-880 (2010).
  6. Gustmann, S., Dunker, N. In vivo-like organotypic murine retinal wholemount culture. J Vis Exp. , (2010).
  7. Franklin, K., Paxinos, G. The Mouse Brain in Stereotaxic Coordinates. , Academic Press. San Diego. (1997).
وسحق العصب البصري النموذجي إصابة الفئران لدراسة الخلايا الشبكية بقاء العقدة
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Cite this Article

Tang, Z., Zhang, S., Lee, C., Kumar, More

Tang, Z., Zhang, S., Lee, C., Kumar, A., Arjunan, P., Li, Y., Zhang, F., Li, X. An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival. J. Vis. Exp. (50), e2685, doi:10.3791/2685 (2011).

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