Materials
Name | Type | Company | Catalog Number | Comments |
Digoxigenin-11-uridine-5’-triphosphate | Reagent | Roche Group | 11209256910 | |
Ribonucleoside Triphosphate Set | Reagent | Roche Group | 11277057001 | |
Quick Spin Columns for radiolabeled RNA purification | Supply | Roche Group | 11274015001 | |
T7 RNA polymerase | Reagent | Roche Group | 10881767001 | |
SP6 RNA polymerase | Reagent | Roche Group | 10810274001 | |
T3 RNA polymerase | Reagent | Roche Group | 11031163001 | |
CTP, [α-32P]- 800Ci/mmol 10mCi/ml , 250 μCi | Reagent | PerkinElmer, Inc. | BLU008X250UC | |
DNase I recombinant, RNase-free | Reagent | Roche Group | 4716728001 | |
Urea,Colorless-to-white Crystals or Crystalline Powder | Reagent | Fisher Scientific | BP169-500 | |
Gel loading buffer | Reagent | Ambion | AM8547 | |
Hybond-N+, Amersham | Supply | GE Healthcare | RPN82B | |
UV Stratalinker 2400 | Equipment | Stratagene, Agilent Technologies | ||
Diethyl pyrocarbonate | Reagent | Sigma-Aldrich | D5758-100ML | |
Heparin sodium | Reagent | Acros Organics | 41121-0010 | |
hydrogen peroxide 30% in water | Reagent | Fisher Scientific | BP2633-500 | |
Gluteraldehyde, 8% EM grade | Reagent | Polysciences, Inc. | 0/710 | Use with caution according to manufacture’s instructions |
Blocking reagent | Reagent | Roche Group | 11096176001 | Make into 5% stock and store at -20° C |
Proteinase K | Reagent | Roche Group | 3115852001 | |
Rnase A | Reagent | Roche Group | 10109142001 | Make as 10 mg/ml stock and store at -20° C. |
Rnase T1 | Reagent | Roche Group | 10109193001 | |
Ultrapure Formamide | Reagent | Invitrogen | 15515-026 | |
Levamisole hydrochloride | Reagent | ICN Biomedicals | 155228 | |
Ribonucleic acid from torula yeast,Type VI | Reagent | Sigma-Aldrich | R6625 | phenol/chloroform extracted several times and precipitated, resuspended in DEPC-dH2O and stored at -20° C |
Anti-Digoxigenin-AP Fab fragments | Reagent | Roche Group | 11093274910 | |
BM Purple AP Substrate, precipitating. Ready-to-use solution. | Reagent | Roche Group | 11 442 074 001 | |
4mL, Clear, Closed Top, Storage Vial Convenience Kit | Supply | National Scientific Company | B7800-2 | |
Shake N Bake hybridization oven | Equipment | Boekel Scientific | 136400 | |
Biopsy Sure-Tek | Supply | Fisher Scientific | 15-200-402C | |
Paraplast Plus tissue embedding medium | Reagent | Fisher Scientific | 23-021-400 | |
Peel-A-Way disposable plastic tissue embedding molds | Supply | Polysciences, Inc. | 18646A | |
Colorfrost Plus Microscope slides | Supply | Fisher Scientific | 12-550-17 | |
Nuclear Fast Red | Reagent | Sigma-Aldrich | N8002 | |
Cytoseal 60 | Reagent | Richard-Allan Scientific | 8310-16 |
References
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