Materials
Name | Company | Catalog Number | Comments |
CD8 T cell isolation kit | Miltenyi Biotec | 130-094-156 | |
Phytohemagglutinin | Remel, Thermo Fisher Scientific | R-30852801 | 2mg/vial |
Yssel’s medium | Gemini Bio Products | 400-102 | 1% pooled human serum |
96-well round bottom plate | BD Biosciences | 08-772-17 | |
Recombinant human IL-2 | R&D Systems | 202-IL-050 | 50μg |
24-well plate | Corning | 07-200-84 | |
Phosphate-buffered saline | Cellgro | MT-21-031-CV | |
Primary anti-IFN-γ monoclonal antibody (D1K) | Mabtech | 3420-3-1000 | |
Biotin-conjugated anti-IFN-γ monoclonal antibody (7B-6) | Mabtech | 3420-6-1000 | |
Multiscreen HA ELISPOT plate | EMD Millipore | MAHA S45 10 | |
Dimethyl Sulphoxide | Sigma-Aldrich | D2650 | 100 ml bottle |
Pooled Serum, human | Atlanta Biologicals | S40510H | Heat inactivate |
RPMI 1640 | Cellgro | MT-10-040-CV | |
Tween-20 | Sigma-Aldrich | P2287-100ml | |
Vectastain Elite ABC Kit | Vector Laboratories | NC9313719 | |
Stable diaminobenzidine | Open Biosystems | MBI 1241 | |
AID EliSpot Reader Classic | Autoimmun Diagnostika GmbH | ELR06 |
References
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