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Medicine

İnsan internal meme arter (İMA) Transplantasyon ve Stent: In-Stent Restenozis Geliştirme Eğitim Bir İnsan Modeli

Published: May 9, 2012 doi: 10.3791/3663

Materials

Name Company Catalog Number Comments
2 French Fogarty catheter Baxter Internationl Inc. 120602F
Yukon Stent Translumina GmbH, Hechingen, Germany Use the stent of your choice according to your study protocol
RPMI media Biochrom AG Nr.F1275
heparin Baxter Internationl Inc. 2B0953
isoflurane Abbott Laboratories B506
Provo-Iodine Betadine Puredue Pharma EAN:5995327165830
80% ethanol Geyer ETV 80/0500
Micro clamp Harvard Apparatus PY2-61-0186
Sutures 8-0 Johnson & Johnson 2808G
Sutures 6-0 Johnson & Johnson 1698 H
Carprofen Feizer Vet PZN:0110208
Metamizol Ratiopharm
Target retrieval solution, pH9 Dako S2368
Image-iT FX signal enhancer Invitrogen I36933
mouse monoclonal anti-GFP antibody BD Biosciences 632381
primary antibody diluent Dako S3022
goat-anti-mouse IgG, Alexa Fluor 488 Invitrogen A11017
secondary antibody diluent Dako S0809
rabbit polycolonal anti-smooth muscle α-actin Abcam ab5694
goat-anti-rabbit IgG, Alexa Fluor 555 Invitrogen A21430
Prolong Gold antifade reagent Invitrogen P36930

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References

  1. Deuse, T., Ikeno, F., Robbins, R. C., Schrepfer, S. Imaging In-Stent Restenosis: An Inexpensive, Reliable, and Rapid Preclinical Model. J. Vis. Exp. (31), e1346 (2009).
  2. Oyamada, S. Trans-iliac rat aorta stenting: a novel high throughput preclinical stent model for restenosis and thrombosis. J. Surg. Res. 166, e91-e95 (2011).
  3. Chamberlain, J. A novel mouse model of in situ stenting. Cardiovascular research. 85, 38-44 (2010).
  4. Deuse, T. Introducing the first polymer-free leflunomide eluting stent. Atherosclerosis. 200, 126-134 (2008).
  5. Finn, A. V. Differential healing after sirolimus, paclitaxel, and bare metal stent placement in combination with peroxisome proliferator-activator receptor gamma agonists: requirement for mTOR/Akt2 in PPARgamma activation. Circulation research. 105, 1003-1012 (2009).
  6. Tellez, A. Coronary bare metal stent implantation in homozygous LDL receptor deficient swine induces a neointimal formation pattern similar to humans. Atherosclerosis. 213, 518-524 (2010).
  7. Suzuki, Y., Yeung, A. C., Ikeno, F. The pre-clinical animal model in the translational research of interventional cardiology. JACC Cardiovasc. Interv. 2, 373-383 (2009).
  8. Holt, C. M. Intimal proliferation in an organ culture of human internal mammary artery. Cardiovascular research. 26, 1189-1194 (1992).
  9. Swanson, N., Javed, Q., Hogrefe, K., Gershlick, A. Human internal mammary artery organ culture model of coronary stenting: a novel investigation of smooth muscle cell response to drug-eluting stents. Clin. Sci. (Lond). 103, 347-353 (2002).
İnsan internal meme arter (İMA) Transplantasyon ve Stent: In-Stent Restenozis Geliştirme Eğitim Bir İnsan Modeli
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Cite this Article

Hua, X., Deuse, T., Michelakis, E.More

Hua, X., Deuse, T., Michelakis, E. D., Haromy, A., Tsao, P. S., Maegdefessel, L., Erben, R. G., Bergow, C., Behnisch, B. B., Reichenspurner, H., Robbins, R. C., Schrepfer, S. Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis. J. Vis. Exp. (63), e3663, doi:10.3791/3663 (2012).

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