Materials
Name | Company | Catalog Number | Comments |
T7 High Yield RNA Synthesis Kit | New England Biolabs | E2040S | |
3’-0-Me-m7G(5’)ppp(5’)G RNA Cap Structure Analog | New England Biolabs | S1411S | |
DNase I (RNase-free) | New England Biolabs | M0303S | |
Vaccinia Capping System | New England Biolabs | M2080S | |
MEGAclear Kit | Ambion | AM1908 | |
NanoDrop Spectrophotometer | Thermo Fisher Scientific, Inc. | ||
Agilent RNA 6000 Nano Kit | Agilent Technologies | 5067-1511 | |
Agilent 2100 Bioanalyzer | Agilent Technologies | G2939AA | |
TransIT mRNA Transfection Kit | Mirus Bio LLC | MIR2250 | |
HyClone* Classical Liquid Media: Dulbecco’s Modified Eagle’s Medium, High Glucose | Thermo Fisher Scientific, Inc. | SH30285.01 | |
HyClone Non-essential Amino Acids | Thermo Fisher Scientific, Inc. | SH30238.01 | |
Centro LB 960 | Berthold Technologies | ||
BioLux Gaussia Luciferase Assay Kit | New England Biolabs | E3300S | |
BioLux Cypridina Luciferase Assay Kit | New England Biolabs | E3309S | |
RNase Inhibitor, Murine | New England Biolabs | M0314 | |
RNase Inhibitor, Human Placenta | New England Biolabs | M0307 | |
pCMV-GLuc Control Plasmid | New England Biolabs | N8081 |
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