Materials
| Name | Company | Catalog Number | Comments |
| T7 High Yield RNA Synthesis Kit | New England Biolabs | E2040S | |
| 3’-0-Me-m7G(5’)ppp(5’)G RNA Cap Structure Analog | New England Biolabs | S1411S | |
| DNase I (RNase-free) | New England Biolabs | M0303S | |
| Vaccinia Capping System | New England Biolabs | M2080S | |
| MEGAclear Kit | Ambion | AM1908 | |
| NanoDrop Spectrophotometer | Thermo Fisher Scientific, Inc. | ||
| Agilent RNA 6000 Nano Kit | Agilent Technologies | 5067-1511 | |
| Agilent 2100 Bioanalyzer | Agilent Technologies | G2939AA | |
| TransIT mRNA Transfection Kit | Mirus Bio LLC | MIR2250 | |
| HyClone* Classical Liquid Media: Dulbecco’s Modified Eagle’s Medium, High Glucose | Thermo Fisher Scientific, Inc. | SH30285.01 | |
| HyClone Non-essential Amino Acids | Thermo Fisher Scientific, Inc. | SH30238.01 | |
| Centro LB 960 | Berthold Technologies | ||
| BioLux Gaussia Luciferase Assay Kit | New England Biolabs | E3300S | |
| BioLux Cypridina Luciferase Assay Kit | New England Biolabs | E3309S | |
| RNase Inhibitor, Murine | New England Biolabs | M0314 | |
| RNase Inhibitor, Human Placenta | New England Biolabs | M0307 | |
| pCMV-GLuc Control Plasmid | New England Biolabs | N8081 |
References
- Lazarev, D., Manley, J. L. Concurrent splicing and transcription are not sufficient to enhance splicing efficiency. RNA. 13, 1546-1557 (2007).
- Donze, O., Picard, D. RNA interference in mammalian cells using siRNAs synthesized with T7. RNA Polymerase. Nucl. Acids Res. 30, 10-46 (2002).
- Van Gelder, R. N. Amplified RNA synthesized from limited quantities of heterogeneous cDNA. Proc. Nati. Acad. Sci. U.S.A. 87, 1663-1667 (1990).
- Schneider, J. Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments. BMC Genomics. 5, 29-29 (2004).
- Conry, R. M. Characterization of a messenger RNA polynucleotide vaccine vector. Cancer Research. 55, 1397-1400 (1995).
- Huang, F., Wang, G., Coleman, T., Li, N. Synthesis of adenosine derivatives as transcription initiators and preparation of 5′ fluorescein- and biotin-labeled RNA through one-step transcription. RNA. 9, 1562-1570 (2003).
- Warren, L. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Cell Stem Cell. 7, 618-630 (2010).
- Furuichi, Y., LaFiandra, A., Shatkin, A. J. 5'-Terminal structure and mRNA stability. Nature. 266, 235-239 (1977).
- Topisirovic, I., Svitkin, Y. V., Sonenberg, N., Shatkin, A. J. Cap and cap binding proteins in the control of gene expression. Wiley Interdisciplinary Reviews. 2, 277-298 (2011).
- Lewis, J. D., Izaurflde, E. The Role of the Cap Structure in RNA Processing and Nuclear Export. Eur. J. Biochem. 247, 461-469 (1997).
- Inoue, K., Ohno, M., Sakamoto, H., Shimura, Y. Effect of the cap structure on pre-mRNA splicing in Xenopus oocyte nuclei. Genes Dev. 3, 1472-1479 (1989).
- Martin, S. A., Paoletti, E., Moss, B. Purification of mRNA guanylyltransferase and mRNA (guanine 7-)methyltransferase from vaccinia virus. J. Biol. Chem. 250, 9322-9329 (1975).
- Martin, S. A., Moss, B. Modification of RNA by mRNA guanylytransferase and mRNA (guanine-7-)methyl-transferase from vaccinia virions. J. Biol. Chem. 250, 9330-9335 (1975).
- Shuman, S., Moss, B. Purification and use of vaccinia virus mRNA capping enzyme. Meth. Enzymol. 181, 170-180 (1990).
- Thiel, V., Herold, J., Scelle, B., Siddell, S. G. Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. J. Gen. Virol. 82, 1273-1281 (2001).
- Marcotrigiano, J., Gingras, A. -C., Sonenberg, N., Burley, S. K. Cocrystal Structure of the Messenger RNA 5′ Cap-Binding Protein (eIF4E) Bound to 7-methyl-GDP. Cell. 89, 951-961 (1997).
- Stepinski, J. Synthesis and properties of mRNAs containing the novel "anti-reverse" cap analogs 7-methyl (3'-O-methyl)GpppG and 7-methyl (3'-deoxy) GpppG. RNA. 7, 1486-1495 (2001).
- Kuge, H., Brownlee, G. G., Gershon, P. D., Richter, J. D. Cap ribose methylation of c-mos mRNA stimulates translation and oocyte maturation in Xenopus laevis. Nucl. Acids Res. 26, 3208-3214 (1998).
- Schagat, T., Paguio, A., Kopish, K. Normalizing Genetic Reporter Assays: Approaches and Considerations for Increasing Consistency and Statistical Significance. Cell Notes. 17, 9-12 (2007).
- Barbosa, E., Moss, B. mRNA (nucleoside-2'-) - methyltransferase from Vaccinia Virus. J. Biol. Chem. 253, 7698-7702 (1978).
- Lockless, S. W. Recognition of capped RNA substrates by VP39, the vaccinia virus-encoded mRNA cap-specific 2'-O-methyltransferase. Biochemistry. 37, 8564-8574 (1998).
