Materials
| Name | Company | Catalog Number | Comments |
| Collagenase I | GIBCO, by Life Technologies | 17100-017 | Use when selecting for T cells |
| Collagenase IV | GIBCO, by Life Technologies | 17104-019 | Use for myeloid selection |
| DNase | Calbiochem | 260913 | |
| RPMI | GIBCO, by Life Technologies | 21870 | |
| Dulbecco’s PBS | Lonza Inc. | 17-5158 | |
| Fetal Bovine Serum | Lonza Inc. | 14-501F | |
| MACs Buffer | 2% FBS for T cells 10% FBS for myeloid cells |
||
| Thy1.1 PE | eBioscience | 551401 | |
| Anti-PE microbeads | Miltenyi Biotec | 120-000-294 | |
| Anti-Pan DC microbeads | Miltenyi Biotec | 120-003-183 | |
| Anti-CD11b microbeads | Miltenyi Biotec | 120-000-300 | |
| LC column | Miltenyi Biotec | 130-042-202 | |
| MS column | Miltenyi Biotec | 130-042-201 |
References
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- Singh, V., Ji, Q., Feigenbaum, L., Leighty, R. M., Hurwitz, A. A. Melanoma progression despite infiltration by in vivo-primed TRP-2-specific T cells. J. Immunother. 32, 129-139 (2009).
- Matheu, M. P., Cahalan, M. D. Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification. J. Vis. Exp. (9), e409 (2007).
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