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Immunology and Infection

En kvantitativ vurdering af Cell Migration af Phagokinetic Track Motilitet Assay

Published: December 4, 2012 doi: 10.3791/4165

Materials

Name Company Catalog Number Comments
Glass Coverslips (15mm) Fisher Scientific 12-545-83
Gelatin 300 Bloom Sigma-Aldrich G-1890
Tetrachloroauric Acid Trihydrate Fisher Chemical G54-1 14.5 mM (a final working solution)
Sodium Citrate Fisher Scientific BP327-500 0.5% (a final working solution)
Paraformaldehyde Fisher Scientific O4042 3% (a final working solution)
100 mm Tissue Culture Dish Sarstedt 83.1802
12-Well Plates Fisher Scientific 08-772-29
24-Well Plates Fisher Scientific 07-200-84
Techne Oven Hybridiser HB-1D LabPlanet 2040500 The standard laboratory oven will suffice
10 ml Serological Pipettes Sarstedt 86.1254.001
Pipet-Aid Filler/Dispenser Drummond 13-681-15
P200 Single-Channel Manual Pipette Rainin PR-200
200 ml Barrier Tips CLP BT200
ImageJ software http://rsb.info.nih.gov/ij/ License: Public Domain
Nikon Eclipse TE300 with a photometrics CoolSNAPfx monochrome 12-bit CCD camera Nikon Discontinued; The most comparable specification has Nikon Eclipse Ti, but a lower end Nikon 80i will be suitable as well. Other brands also provide comparable microscopes.
Note: The reagents and equipment listed below have been utilized by us in our various studies. Other supplies, suppliers, reagents, and equipment can be used, as long as they have similar specifications.

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References

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En kvantitativ vurdering af Cell Migration af Phagokinetic Track Motilitet Assay
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Cite this Article

Nogalski, M. T., Chan, G. C. T.,More

Nogalski, M. T., Chan, G. C. T., Stevenson, E. V., Collins-McMillen, D. K., Yurochko, A. D. A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay. J. Vis. Exp. (70), e4165, doi:10.3791/4165 (2012).

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