Materials
| Name | Company | Catalog Number | Comments |
| Materials: | |||
| Necessary equipment for raising fish and collecting eggs (see the Zebrafish Book22 for details). | |||
| Stereomicroscope | Leica | Lecia MZ6 Stereomicroscope | Equipped with laser operating at 488 nm |
| Confocal laser scanning microscope | Zeiss | Zeiss LSM 710 NLO | equipped with lasers operating at 488 nm, 561 nm, and 405 nm |
| Heating block | |||
| Pair of Dumont #5 forceps | |||
| Cover-glass-bottomed culture dish | MatTek Corp | Ashland MA USA | |
| Microloader Pipette Tips | Eppendorf | ||
| Vortex | |||
| needles | |||
| Reagents: | |||
| Egg water medium | |||
| 60 μg/ml Instant Ocean Sea Salts in ddH2O | |||
| E3 medium | |||
| 5 mM NaCl | |||
| 0.17 mM KCl | |||
| 0.33 mM CaCl2 | |||
| 0.33 mM MgSO4 | |||
| 0.00001% (w/v) Methylene Blue | |||
| 4 mg/ml Tricaine stock solution | |||
| 400 mg Tricaine (3-amino benzoic acidethylester) | Sigma-Aldrich | ||
| 97.9 ml ddH2O | |||
| 2.1 ml 1M Tris (pH9) | |||
| Adjust at pH 7, aliquot and store -20 °C | |||
| 1% LMA (Low melting temperature agarose, Lonza) | |||
| 1g LMA/100 ml E3 medium 1X | |||
| Aliquot and store at 4 °C | |||
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