Materials
Name | Company | Catalog Number | Comments |
Name of reagent |
Company | Catalog number |
Comments |
6-8 week old female mice |
Jackson Laboratories | Strain Name: C57BL/6J Stock Number: 000664 |
|
Trypsin* |
MediaTech | MT25-053-CI |
Obtained through Fisher |
DMEM* |
MediaTech | MT10-017-CV |
Obtained through Fisher |
FBS |
Hyclone | SH30071.03 |
Heat-inactivated |
T25 flasks* |
Corning Costar | Corning No.:3056 Fisher: 07-200-63 |
Obtained through Fisher |
MB49 cells |
N/A | N/A |
Obtained from Dr. Boehle (see reference11) |
Puralube Vet Ointment* |
Pharmaderm | Henry Schein Company No.:036090-6050059 Fisher: NC9676869 |
Obtained through Fisher |
Depilatory cream: Veet |
local pharmacy | ||
Lubricant: K-Y Jelly |
local pharmacy | ||
Catheters* |
Exel International | Exel International No.:26751; Fisher: 14-841-21 |
Obtained through Fisher |
Isoflurane |
Terrell | NDC 66794-011-25 |
Obtained though hospital pharmacy |
1 ml slip tip TB syringes |
Becton Dickinson | BD309659 Fisher:14-823-434 |
|
D-Luciferin |
Gold Biotechnologies | L-123-1 |
|
Ad-CMV-Luc |
VectorBiolabs | 1000; Request large scale amplification and CsCl purification for in vivo use |
Infectious agent that requires BSL2 containment |
Steady-Glo Luciferase Assay System |
Promega | E2510 (10 ml), E2520 (100 ml), or E2550 (10 x 100 ml) |
|
*available through multiple vendors |
|||
EQUIPMENT |
|||
Anesthesia system |
E-Z Systems, Euthanex Corporation | Anesthesia system: EZ7000 5-port mouse rebreathing device: EZ109 |
Obtained through Fisher |
Xenogen IVIS 200 |
Caliper Life Sciences | http://www.caliperls.com/products/preclinical-imaging/ivis-imaging-system-200-series.htm |
|
FLUOstar Optima |
BMG Labtech | http://www.bmglabtech.com/products/microplate-reader/instruments.cfm?product_id=2 |
References
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