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Medindo o transporte de catiões de Na, K e H, K-ATPase em Published: February 19, 2013 doi: 10.3791/50201


Name Company Catalog Number Comments
4.0 Ethicon Vicryl suture material Johnson Johnson V633H
Collagenase type 1A from Clostridium hystolyticum Sigma Aldrich C9891
High-Pure PCR Product Purification Kit Roche Applied Science 11732676001
Nuclease-free water Ambion AM9937
mMessage mMachine Kit SP6/T7 Ambion 1340, 1344
Ouabain octahydrate Sigma Aldrich O3125
Tricain (ethyl 3-aminobenzoate methanesulfonate salt) Sigma Aldrich A5040
Trypsin inhibitor type III-O from chicken egg white Sigma Aldrich T2011
SCH28080 Sigma Aldrich S4443
AAnalyst 800 Perkin Elmer 0993-5256
WinLab32TM Perkin Elmer
BioPhotometer Eppendorf 6131 000.012
Borosilicate Capillaries Science Products GB150F-8P
Hematocrit tubes 3.5" Drummond Scientific 3 000-203-G/X
Hollow Cathode Lamp Lithium Photron P929LL
Hollow Cathode Lamp Rubidium Photron P945
Micropipette Puller Narishige Model PC-10
Oocyte Recording Chamber RC-10 Warner Instr. W4 64-0306
Nanoject II Injection Pump Drummond Scientific 3-000-204
pCLAMP software Molecular Devices
Polypropylene Sample Cup (1.2 ml) Perkin Elmer B0510397
Speedvac - Concentrator model 5301 Eppendorf 5301 000.210
THGA Tube Perkin Elmer B3000641
Turbo TEC-10CX Amplifier NPI Electronics TEC-10CX



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Medindo o transporte de catiões de Na, K e H, K-ATPase em<em&gt; Xenopus</em&gt; Oócitos por espectrofotometria de absorção atômica: uma alternativa para Ensaios de Radioisótopos
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Dürr, K. L., Tavraz, N. N., Spiller, S., Friedrich, T. Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays. J. Vis. Exp. (72), e50201, doi:10.3791/50201 (2013).More

Dürr, K. L., Tavraz, N. N., Spiller, S., Friedrich, T. Measuring Cation Transport by Na,K- and H,K-ATPase in Xenopus Oocytes by Atomic Absorption Spectrophotometry: An Alternative to Radioisotope Assays. J. Vis. Exp. (72), e50201, doi:10.3791/50201 (2013).

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