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JoVE Journal Immunology and Infection
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Immunology and Infection

PrP som en ny tilnærming for å hindre smitte: Forberedelse og Published: April 9, 2013 doi: 10.3791/50351
Automatic Translation
1,2, 1,3,4
1Department of Orthopaedics, School of Medicine, West Virginia University, 2Department of Orthopaedics, Stem Cell Research Center, University of Pittsburgh, 3WVNano Initiative, 4Mary Babb Randolph Cancer Center

Abstract

Implantat-assosiert infeksjon blir mer og mer utfordrende til helsevesenet over hele verden på grunn av økende antibiotikaresistens, overføring av antibiotikaresistente bakterier mellom dyr og mennesker, og de høye kostnadene ved behandling av infeksjoner.

I denne studien, utleverer vi en ny strategi som kan være effektive i å forebygge implantat-assosiert infeksjon basert på de potensielle antimikrobielle egenskapene blodplate-rik plasma (PRP). På grunn av sin veloverveide egenskaper for å fremme helbredelse, har PRP (et biologisk produkt) blitt stadig mer brukt for kliniske applikasjoner, inkludert ortopediske operasjoner, periodontal og muntlig operasjoner, maxillofacial operasjoner, plastisk kirurgi, idrettsmedisin, osv.

PRP kan være et avansert alternativ til konvensjonelle antibiotika behandlinger i å forebygge implantat-infeksjoner. Bruken av PRP kan være fordelaktig i forhold til konvensjonelle antibiotika behandlinger since PRP er mindre sannsynlig å indusere antibiotikaresistens og PRP er antimikrobiell og helbredende-fremme egenskaper kan ha en synergistisk effekt på infeksjon forebygging. Det er vel kjent at patogener og humane celler er racing for implantasjon overflater, og PRP egenskaper for å fremme helbredelse kan forbedre human cellefesting dermed redusere sjanser for infeksjon. I tillegg er PRP iboende biokompatibel, og sikker og fri fra risikoen av overførbare sykdommer.

For vår studie har vi valgt flere kliniske bakteriestammer som vanligvis finnes i ortopediske infeksjoner og undersøkt om PRP har in vitro antimikrobielle egenskaper mot disse bakteriene. Vi har utarbeidet PRP ved hjelp av en dobbelt sentrifugering tilnærming som tillater den samme blodplater konsentrasjonen som skal oppnås for alle prøver. Vi har oppnådd konsekvent antimikrobielle funn og fant ut at PRP har sterk in vitro antimikrobielle egenskaper mot bakterier som methicillin-sensitive og meticillinresistente Staphylococcus aureus, gruppe A streptokokker, og Neisseria gonorrhoeae. Derfor kan bruken av PRP har potensial for å hindre smitte og å redusere behovet for kostbare postoperativ behandling av implantat-assosierte infeksjoner.

Materials

Name Company Catalog Number Comments
Bovine thrombin King Pharmaceuticals, Inc 60793-215-05 Thrombin (bovine origin)
Calcium chloride King Pharmaceuticals, Inc 60793-215-05 10% calcium chloride
Ethanol Sigma-Aldrich E7023
Isoflurane Baxter 1001936060
Mueller Hinton broth Becton, Dickinson and Company 275710
Phosphate-buffered saline Sigma-Aldrich D8662
Tri-sodium citrate Sigma-Aldrich W302600
Tryptic soy agar Fisher Scientific R01202
Centrifuge Kendro Laboratory Products 750043077
Syringe filter Millipore SLGP033RS

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PrP som en ny tilnærming for å hindre smitte: Forberedelse og<em&gt; In vitro</em&gt; Antimikrobielle egenskaper av PrP
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Li, H., Li, B. PRP as a New Approach … More

Li, H., Li, B. PRP as a New Approach to Prevent Infection: Preparation and In vitro Antimicrobial Properties of PRP. J. Vis. Exp. (74), e50351, doi:10.3791/50351 (2013).

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