Materials
Name | Company | Catalog Number | Comments |
Materials | |||
Rat Anti-mouse CD11b | Kindly provided by Dr. A. Doni, Istituto Clinico Humanitas, Milan, Italy | ||
Rat Anti-mouse CD68 | AbD Serotec | MCA 1957 | |
Rabbit Anti-mouse Ym1 | Stem Cell Technologies | 1404 | |
H–chst 33342 | Life technologies | H21492 | |
Mouse Anti-Neural Nuclei (NeuN) | CHEMICON | MAB377 | |
Biotinilated Goat Anti-Rat antibody | Jackson Immuno Research | 112-065-143 | |
TSA Cyanine 5 System | Perkin Elmer | NEL705A001KT | |
Prolong Gold | Invitrogen | P36930 | |
Anti-rat alexa 546 | Invitrogen | A-11081 | |
Anti mouse Alexa 488 | Invitrogen | A-21121 | |
Anti-rabbit Alexa 594 | Invitrogen | A-11037 | |
Normal Goat Serum | Vectors Laboratories | S-1000 | |
Tritin X-100 | Sigma | T8787 | |
Phosphate Buffered Saline | Sigma | P4417-100 | |
Equipment | |||
Cryostat CM1850 | Leica | ||
Olympus IX81 confocal microscope | Olympus | ||
AnalySIS software | Olympus | ||
Imaris software 5.0 | Bitplane | ||
Photoshop cs2 | Adobe Systems | ||
Software packages GraphPad Prism version 4.0 | GraphPad Software Inc. |
References
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