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Neuroscience

İskemik Hücre Ölümü Pathways Eğitim için seks Katmanlı Nöronal Kültürleri

Published: December 9, 2013 doi: 10.3791/50758
Automatic Translation
*1, *1, 2, 1,3, 1,3
1Department of Anesthesiology, University of Colorado School of Medicine, 2Oregon National Primate Research Center, Oregon Health & Science University, 3Department of Pharmacology, University of Colorado School of Medicine
* These authors contributed equally

Materials

Name Company Catalog Number Comments
B27 Supplement Invitrogen 17504-044
Borax Sigma 71997
Boric Acid Sigma B6768
Bovine Serum Albumin Sigma A4503
Fetal Bovine Serum Invitrogen 26140-079
GlutaMAX Invitrogen 35050-061
Glutamine Invitrogen 25030-081
HBSS, no calcium, no magnesium, no phenol red Invitrogen 14175-095
MEM, no glutamine, no phenol red Invitrogen 51200-038
Neurobasal, no glutamine, no phenol red Invitrogen 12348-017
Penicillin/Streptomycin Invitrogen 15070-063
Poly-D-lysine, MW 70,000-150,000 Sigma P0899
Trypan Blue 0.4% Invitrogen 15250-061
EQUIPMENT
Dumont #5/45 Angled Forceps Fine Science Tools 11251-35
Dumont #7 – Fine Curved Forceps Fine Science Tools 11274-20
Serrated (London) Forceps Fine Science Tools 11080-02
Extra Fine Graefe Forceps 1 x 2 teeth Fine Science Tools 11153-10
Moria Spring Scissors Fine Science Tools 15396-00
Medium Operating Scissors Roboz Surgical RS-6812
Extra Fine Microdissecting Scissors Roboz Surgical RS-5880
24-well Tissue Culture Plates Fisher 87721
Cell Strainer, 40 µM Nylon Mesh BD Biosciences 352340

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References

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İskemik Hücre Ölümü Pathways Eğitim için seks Katmanlı Nöronal Kültürleri
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Cite this Article

Fairbanks, S. L., Vest, R., Verma,… More

Fairbanks, S. L., Vest, R., Verma, S., Traystman, R. J., Herson, P. S. Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways. J. Vis. Exp. (82), e50758, doi:10.3791/50758 (2013).

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