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May 2013: This Month in JoVE

Published: May 1, 2013 doi: 10.3791/5080
1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production


Here are some highlights from the May 2013 Issue of Journal of Visualized Experiments (JoVE).


Minimal Erythema Dose (MED) Testing

Carolyn J. Heckman1, Rachel Chandler2, Jacqueline D. Kloss3, Amy Benson2, Deborah Rooney2, Teja Munshi1, Susan D. Darlow1, Clifford Perlis4, Sharon L. Manne5, David W. Oslin2

1Cancer Prevention and Control Program, Fox Chase Cancer Center, 2Department of Psychiatry, University of Pennsylvania, 3Department of Psychology, Drexel University, 4Department of Medicine, Fox Chase Cancer Canter, 5Cancer Prevention and Control Program, The Cancer Institute of New Jersey

This article describes how to conduct minimal erythema dose (MED) testing in order to determine the lowest dose of ultraviolet radiation that will cause erythema (burning) when administered to an individual.

Measuring Intracellular Ca2+ Changes in Human Sperm Using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Esperanza Mata-Martínez1, Omar José1, Paulina Torres-Rodríguez1, Alejandra Solís-López1, Ana A. Sánchez-Tusie1, Yoloxochitl Sánchez-Guevara1, Marcela B. Treviño2, Claudia L. Treviño1

1Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología-Universidad Nacional Autónoma de México, 2Math and Sciences Department, Edison State College

Intracellular Ca2+ dynamics are very important in sperm physiology and Ca2+-sensitive fluorescent dyes constitute a versatile tool to study them. Population experiments (fluorometry and stopped flow fluorometry) and single cell experiments (flow cytometry and single cell imaging) are used to track spatio-temporal [Ca2+] changes in human sperm cells.

Air Filter Devices Including Nonwoven Meshes of Electrospun Recombinant Spider Silk Proteins

Gregor Lang, Stephan Jokisch, Thomas Scheibel

Biomaterials Research Group, University of Bayreuth

Spider silk fibers display extraordinary mechanical properties. Engineered Araneus diadematus Fibroin 4 (eADF4) can be processed into nonwoven meshes using electrospinning. Here, the eADF4 nonwoven meshes are used to improve the performance of air filtering devices.

Ex vivo Live Imaging of Single Cell Divisions in Mouse Neuroepithelium

Karolina Piotrowska-Nitsche1,2, Tamara Caspary1

1Department of Human Genetics, Emory University School of Medicine, 2Department of Experimental Embryology, IGAB Polish Academy of Sciences

Here we develop the tools necessary for ex vivo live imaging to trace single cell divisions in the mouse E8.5 neuroepithelium

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Chun A. Changou1, Deanna L. Wolfson2, Balpreet Singh Ahluwalia3, Richard J. Bold4, Hsing-Jien Kung5, Frank Y.S. Chuang6

1Department of Biochemistry and Molecular Medicine, NSF Center for Biophotonics Science & Technology, 2NSF Center for Biophotonics Science & Technology, University of California, Davis, 3NSF Center for Biophotonics Science & Technology, University of Tromsø, 4Department of Surgery, University of California, Davis, 5Department of Biological Chemistry, University of California, Davis, 6Department of Biochemistry and Molecular Medicine, University of California, Davis

Autophagy is a ubiquitous process that enables cells to degrade and recycle proteins and organelles. We apply advanced fluorescence microscopy to visualize and quantify the small, but essential, physical changes associated with the induction of autophagy, including the formation and distribution of autophagosomes and lysosomes, and their fusion into autolysosomes.


No conflicts of interest declared.


May 2013: This Month in JoVE
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Chao, W., Kolski-Andreaco, A. MayMore

Chao, W., Kolski-Andreaco, A. May 2013: This Month in JoVE. J. Vis. Exp. (75), e5080, doi:10.3791/5080 (2013).

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