Materials
Name | Company | Catalog Number | Comments |
Sodium nitroprusside | Sigma-Aldrich | 71778-25G | |
Heparin sodium salt | Fisher | BP2425 | |
Triton X-100 | Fisher | BP151-100 | |
Sodium deoxycholate | Fisher | BP349-100 | |
Sodium chloride | Fisher | 7647-14-5 | |
Bovine pancreatic DNase | Sigma-Aldrich | DN25 | Prepare stock, aliquot, and freeze |
Magnesium sulfate | Fisher | 10034-99-8 | |
Calcium chloride | Fisher | C614-500 | |
PBS (no Ca/Mg) | Gibco, Life Technologies | 10010-031 | |
Antibiotic-Antimycotic | Gibco Life Technologies | 15240062 | |
Cell Culture Media | |||
Alpha MEM | Gibco Life Technologies | 12561-072 | |
Medium 199 | Gibco Life Technologies | 11150067 | |
Premium Fetal Bovine Serum | Atlanta Biologicals | S11150 | |
L-Glutamine 100x | Gibco Life Technologies | 25030-081 | |
Endothelial Cell Growth Supplement (ECGS) | ScienCell | 1052 | |
Antibiotic-Antimycotic | Gibco Life Technologies | 15240062 | |
Cell Seeding and Bioreactor Culture | |||
Check valves | Cole-Parmer | EW-98553-20 | |
Y-connectors | Cole-Parmer | ED-30614-08 | |
3-Way stopcocks | Harvard Apparatus | 721664 | |
MasterFlex L/S 14 tubing | Cole-Parmer | 96420-14 | |
MasterFlex L/S 16 tubing | Cole-Parmer | 96420-16 | |
Male lock Luer 1/8 in | Cole-Parmer | EW-45505-04 | |
Female Luer 1/8 in | Cole-Parmer | SI-45502-04 | |
Male Luer lock plug | Cole-Parmer | EW-45505-56 | |
Injection ports | Medi-Dose EPS | IV2004 | |
Latex tubing | St. Louis Medical Suppy | HN10910 | |
Hose clamp | Cole-Parmer | EW-06832-02 | |
2 L Wide-mouth jar | Fisher | 05-719-276 | |
60 ml Syringes | Fisher | NC9035364 |
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