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Biology

Gebruik Published: December 18, 2013 doi: 10.3791/50840
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1, 1, 1, 1, 1, 1
1Department of Life Sciences, National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev

Materials

Name Company Catalog Number Comments
Antibody staning and fiaxtion paraformaldehyde Merc 8187151000
Tween-20  Bio-Rad 1706531
Collagenase Sigma C5138-100MG
DABCO Merc 8034560100
Triton X-100 Alfa Aesar A16046
β -ME Sigma 8057400250
BSA Di Cam 000-40-100
 Partial digest  Chymotrypsin Sigma C4129-250MG
Thermo-resistance SYTOX Orange Invitrogen S11368
Cholesterol Amaresco 0433-250G
EQUIPMENT
M165 FC fluorescent stereoscope Leica TXR filter
EXi Blue Fluorescence Microscopy Camera QImaging EXI-BLU-R-F-M-14-C
ConfoCor 3/510 META confocal microscope Zeiss
Pellet pestles Sigma Z359947-100EA
Pellet Pestle Cordless Motor Kontes K749540-0000
MicroCL 17 Microcentrifuge Series Thermo 75002455 Refrigerated, , 230 V 50/60 Hz, includes 24x 1.5/2.0 ml rotor with ClickSeal Biocontainment Lid

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References

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Gebruik<em&gt; Caenorhabditis elegans</em&gt; Als een modelsysteem voor de Protein Homeostase studeren in een meercellige
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Cite this Article

Karady, I., Frumkin, A., Dror, S.,… More

Karady, I., Frumkin, A., Dror, S., Shemesh, N., Shai, N., Ben-Zvi, A. Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism. J. Vis. Exp. (82), e50840, doi:10.3791/50840 (2013).

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