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Neuroscience

Functionele Beeldvorming van de auditieve cortex in volwassen katten met behulp van High-field fMRI

Published: February 19, 2014 doi: 10.3791/50872
Automatic Translation
1,6, 5, 5, 1,6, 3,4,5, 1,2,4,5,6,7
1Department of Physiology and Pharmacology, University of Western Ontario, 2Department of Psychology, University of Western Ontario, 3Department of Medical Biophysics, University of Western Ontario, 4Brain and Mind Institute, University of Western Ontario, 5Centre for Functional and Metabolic Mapping, Robarts Research Institute, University of Western Ontario, 6Cerebral Systems Laboratory, University of Western Ontario, 7National Centre for Audiology, University of Western Ontario

Abstract

De huidige kennis van sensorische verwerking in het auditief systeem van zoogdieren is voornamelijk afkomstig van elektrofysiologische studies in verschillende diermodellen, zoals apen, fretten, knuppels, knaagdieren en katten. Om geschikte parallellen tussen menselijke en dierlijke modellen van gehoorfunctie trekken, is het belangrijk om een ​​brug tussen menselijke functionele beeldvorming studies en dierlijke elektrofysiologische studies stellen. Functionele magnetische resonantie imaging (fMRI) is een gevestigde, minimaal invasieve methode voor het meten brede patronen van hemodynamische activiteit in verschillende gebieden van de cerebrale cortex. Deze techniek wordt veel gebruikt voor sensorische functie in het menselijk brein sonde is een nuttig instrument koppelen studies auditieve verwerking bij mens en dier en is met succes gebruikt om gehoorfunctie bij apen en knaagdieren onderzoeken. Het volgende protocol beschrijft een experimentele procedure voor het onderzoeken van auditieve functie in verdoofde volwassenkatten door het meten-stimulus opgeroepen hemodynamische veranderingen in de auditieve cortex met behulp van fMRI. Deze methode maakt vergelijking van de hemodynamische responsen in verschillende modellen gehoorfunctie hetgeen leidt tot een beter begrip van species-onafhankelijke eigenschappen van de zoogdieren auditieve cortex.

Materials

Name Company Catalog Number Comments
Materials
Atropine sulphate injection 0.5 mg/ml Rafter 8 Products
Acepromazine 5 mg/ml Vetoquinol Inc.
Ketamine hydrochloride 100 mg/ml Bimeda-MTC
Dexmedetomidine hydrochloride (Dexdomitor 0.5 mg/ml) Orion Pharma
Isoflurane 99.9% Abbott Laboratories
Lidocaine (Xylocaine endotracheal 10 mg/metered dose) Astra Zeneca
Lubricating opthalmic ointment (Refresh Lacri Lube) Allergan Inc.
Saline 0.95%
IV Catheter 22 g (wings)
IV Extension Set Codan US Corp. BC 269
IV Administration Set 10 drips/ml
Endotracheal tube 4.0
Heating pads (Snuggle Safe) Lenric C21 Ltd.
Syringe 60 ml
Equipment
External sound card Roland Corporation Cakewalk UA-25EX
Stereo power amplifier Pyle Audio Inc. Pyle Pro PCAU11
MRI-compatible insert earphone system Sensimetric Corporation Model S14
Foam ear tips for insert earphones E-A-R Auditory Systems Earlink 3B
End-tidal CO2 monitor Nellcor N-85
MRI-compatible pulse oximeter Nonin Medical Inc. Model 7500
Syringe pump Harvard Apparatus 70-2208

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References

  1. Kaas, J. H., Hackett, T. A. Subdivisions of auditory cortex and processing streams in primates. Proc. Natl. Acad. Sci. U.S.A. 97, 11793-11799 (2000).
  2. Kusmierek, P., Rauschecker, J. P. Functional Specialization of Medial Auditory Belt Cortex inthe Alert Rhesus Monkey. J. Neurophysiol. 102, 1606-1622 (2009).
  3. Recanzone, G. H., Guard, D. C., Phan, M. L. Frequency and Intensity Response Properties of Single Neurons in the Auditory Cortex of the Behaving Macaque Monkey. J. Neurophysiol. 83, 2315-2331 (2000).
  4. Godey, B., Atencio, C. A., Bonham, B. H., Schreiner, C. E., Cheung, S. W. Functional organization of squirrel monkey primary auditory cortex: Responses to frequency-modulation sweeps. J. Neurophysiol. 94, 1299-1311 (2005).
  5. Tian, B., Rauschecker, J. P. Processing of frequency-modulated sounds in the lateral auditory belt cortex of the rhesus monkey. J. Neurophysiol. 92, 2993-3013 (2004).
  6. Mrsic-Flogel, T. D., Versnel, H., King, A. J. Development of contralateral and ipsilateral frequency representations in ferret primary auditory cortex. Eur. J. Neurosci. 23, 780-792 (2006).
  7. Elhilali, M., Fritz, J. B., Chi, T. -S., Shamma, S. A. Auditory Cortical Receptive Fields: Stable Entities with Plastic Abilities. J. Neurosci. 27, 10372-10382 (2007).
  8. Shamma, S. A., Fleshman, J. W., Wiser, P. R., Versnel, H. Organization of Response Areas in Ferret Primary Auditory Cortex. J. Neurophysiol. 69, 367-383 (1993).
  9. Kowalski, N., Versnel, H., Shamma, S. A. Comparison of Responses in the Anterior and Primary Auditory Fields of the Ferret Cortex. J. Neurophysiol. 73, 1513-1523 (1995).
  10. Nelken, I., Versnel, H. Responses to linear and logarithmic frequency-modulated sweeps in ferret primary auditory cortex. Eur. J. Neurosci. 12, 549-562 (2000).
  11. Shannon-Hartman, S., Wong, D., Maekawa, M. Processing Of Pure-Tone And FM Stimuli In The Auditory Cortex Of The FM Bat, Myotis lucifugus. Hearing Res. 61, 179-188 (1992).
  12. Razak, K. A., Fuzessery, Z. M. Neural Mechanisms Underlying Sensitivity for the Rate and Direction of Frequency-Modulated Sweeps in the Auditory Cortex of the Pallid. J. Neurophysiol. 96, 1303-1319 (2006).
  13. Razak, K. A., Fuzessery, Z. M. GABA Shapes Selectivity for the Rate and Direction of Frequency-Modulated Sweeps in the Auditory Cortex. J. Neurophysiol. 102, 1366-1378 (2009).
  14. Suga, N. Functional Properties of Auditory Neurones in the Cortex of Echo-Locating Bats. J. Physiol. 181, 671-700 (1965).
  15. Harrison, R. V., Kakigi, A., Hirakawa, H., Harel, N., Mount, R. J. Tonotopic mapping in auditory cortex of chinchilla. Hearing Res. 100, 157-163 (1996).
  16. Benson, D. A., Teas, D. C. Single Unit Study of Binaural Interaction in the Auditory Cortex of the Chinchilla. Brain Res. 103, 313-338 (1976).
  17. Ricketts, C., Mendelson, J. R., Anand, B., English, R. Responses to time-varying stimuli in rat auditory cortex. Hearing Res. 123, 27-30 (1998).
  18. Gaese, B. H., Ostwald, J. Temporal Coding of Amplitude and Frequency Modulation in the Rat Auditory Cortex. European J. Neurosci. 7, 438-450 (1995).
  19. Hage, S. R., Ehret, G. Mapping responses to frequency sweeps and tones in the inferior colliculus of house mice. Eur. J. Neurosci. 18, 2301-2312 (2003).
  20. Merzenich, M. M., Knight, P. L., Roth, G. L. Representation of Cochlea Within Primary Auditory Cortex in the Cat. J. Neurophysiol. 38, 231-249 (1975).
  21. Knight, P. L. Representation of the Cochlea within the Anterior Auditory Field (AAF) of the Cat. Brain Res. 130, 447-467 (1977).
  22. Sutter, M. L., Schreiner, C. E., McLean, M., O'Connor, K. N., Loftus, W. C. Organization of Inhibitory Frequency Receptive Fields in Cat Primary Auditory Cortex. J. Neurophysiol. 82, 2358-2371 (1999).
  23. Whitfield, I. C., Evans, E. F. Responses of Auditory Cortical Neurons to Stimuli of Changing Frequency. J. Neurophysiol. 28, 655-672 (1965).
  24. Mendelson, J. R., Cynader, M. S. Sensitivity of cat primary auditory cortex (AI) neurons to the direction and rate of frequency modulation. Brain Res. 327, 331-335 (1985).
  25. Pouratian, N., Toga, A. W. Brain Mapping: The Methods. Toga, A. W., Mazziotta, J. C. , Academic Press. 97-140 (2002).
  26. Harel, N., Lee, S. P., Nagaoka, T., Kim, D. S., Kim, S. G. Origin of negative blood oxygenation level-dependent fMRI signals. J. Cereb. Blood Flow Metab. 22, 908-917 (2002).
  27. Olman, C., Ronen, I., Ugurbil, K., Kim, D. S. Retinotopic mapping in cat visual cortex using high-field functional magnetic resonance imaging. J. Neurosci. Methods. 131, 161-170 (2003).
  28. Kim, D. S., Duong, T. Q., Kim, S. G. High-resolution mapping of iso-orientation columns by fMRI. Nat. Neurosci. 3, 164-169 (2000).
  29. Belin, P., Zatorre, R. J., Hoge, R., Evans, A. C., Pike, B. Event-related fMRI of the auditory cortex. NeuroImage. 10, 417-429 (1999).
  30. Logothetis, N. K., Pauls, J., Augath, M., Trinath, T., Oeltermann, A. Neurophysiological investigation of the basis of the fMRI signal. Nature. 412, 150-157 (2001).
  31. Mukamel, R., et al. Coupling between neuronal firing, field potentials, and fMR1 in human auditory cortex. Science. 309, 951-954 (2005).
  32. Bilecen, D., Scheffler, K., Schmid, N., Tschopp, K., Seelig, J. Tonotopic organization of the human auditory cortex as detected by BOLD-FMRI. Hearing Res. 126, 19-27 (1998).
  33. Talavage, T. M., Ledden, P. J., Benson, R. R., Rosen, B. R., Melcher, J. R. Frequency-dependent responses exhibited by multiple regions in human auditory cortex. Hearing Res. 150, 225-244 (2000).
  34. Talavage, T. M., et al. Tonotopic organization in human auditory cortex revealed by progressions of frequency sensitivity. J. Neurophysiol. 91, 1282-1296 (2004).
  35. Wessinger, C. M., Buonocore, M. H., Kussmaul, C. L., Mangun, G. R. Tonotopy in human auditory cortex examined with functional magnetic resonance imaging. Human Brain Map. 5, 18-25 (1997).
  36. Cheung, M. M., et al. BOLD fMRI investigation of the rat auditory pathway and tonotopic organization. NeuroImage. 60, 1205-1211 (2012).
  37. Langers, D. R. M., van Dijk, P., Backes, W. H. Lateralization connectivity and plasticity in the human central auditory system. NeuroImage. 28, 490-499 (2005).
  38. Petkov, C. I., Kayser, C., Augath, M., Logothetis, N. K. Functional imaging reveals numerous fields in the monkey auditory cortex. PLoS Biol. 4, 1213-1226 (2006).
  39. Tanji, K., et al. Effect of sound intensity on tonotopic fMRI maps in the unanesthetized monkey. NeuroImage. 49, 150-157 (2010).
  40. Zhang, J. W., et al. Functional magnetic resonance imaging of sound pressure level encoding in the rat central auditory system. NeuroImage. 65, 119-126 (2013).
  41. Baumann, S., et al. Characterisation of the BOLD response time course at different levels of the auditory pathway in non-human primates. NeuroImage. 50, 1099-1108 (2010).
  42. Jezzard, P., Rauschecker, J. P., Malonek, D. An in vivo model for functional MRI in cat visual cortex. Magn. Reson. Med. 38, 699-705 (1997).
  43. Brown, T. A., et al. Characterisation of the blood-oxygen level-dependent (BOLD) response in cat auditory cortex using high-field fMRI. NeuroImage. 64, 458-465 (2013).
  44. Olfert, E. D., Cross, B. M., McWilliam, A. A. Canadian Council on Animal Care. 1, Ottawa, Ontario. (1993).
  45. Franceschini, M. A., et al. The effect of different anesthetics on neurovascular coupling. NeuroImage. 51, 1367-1377 (2010).
  46. Heil, P., Irvine, D. R. F. Functional specialization in auditory cortex: Responses to frequency-modulated stimuli in the cat's posterior auditory field. J. Neurophysiol. 79, 3041-3059 (1998).
  47. Norena, A. J., Gourevitch, B., Pienkowski, M., Shaw, G., Eggermont, J. J. Increasing spectrotemporal sound density reveals an octave-based organization in cat primary auditory cortex. J. Neurosci. 28, 8885-8896 (2008).
  48. Pienkowski, M., Eggermont, J. J. Long-term, partially-reversible reorganization of frequency tuning in mature cat primary auditory cortex can be induced by passive exposure to moderate-level sounds. Hearing Res. 257, 24-40 (2009).
  49. Zurita, P., Villa, A. E. P., de Ribaupierre, Y., de Ribaupierre, F., Rouiller, E. M. Changes of single unit activity in the cat auditory thalamus and cortex associated with different anesthetic conditions. Neurosci. Res. 19, 303-316 (1994).
  50. Crosby, G., Crane, A. M., Sokoloff, L. Local changes in cerebral glucose-utilization during ketamine anesthesia. Anesthesiology. 56, 437-443 (1982).
  51. Zhao, F., Jin, T., Wang, P., Kim, S. -G. Isoflurane anesthesia effect in functional imaging studies. NeuroImage. 38, 3-4 (2007).
  52. Cheung, S. W., et al. Auditory cortical neuron response differences under isoflurane versus pentobarbital anesthesia. Hearing Res. 156, 115-127 (2001).
  53. Dueck, M. H., et al. Propofol attenuates responses of the auditory cortex to acoustic stimulation in a dose-dependent manner: A FMRI study. Acta Anaesthesiol. Scand. 49, 784-791 (2005).
  54. Seifritz, E., et al. Spatiotemporal pattern of neural processing in the human auditory cortex. Science. 297, 1706-1708 (2002).
  55. Hall, D. A., et al. 34;Sparse" temporal sampling in auditory fMRI. Human Brain Map. 7, 213-223 (1999).
  56. Backes, W. H., van Dijk, P. Simultaneous sampling of event-related BOLD responses in auditory cortex and brainstem. Magn. Reson. Med. 47, 90-96 (2002).
  57. Greene, S. A. In Veterinary Anesthesia and Pain Management Secrets. , Hanley & Belfus, Inc. 121-126 (2002).
Functionele Beeldvorming van de auditieve cortex in volwassen katten met behulp van High-field fMRI
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Brown, T. A., Gati, J. S., Hughes,… More

Brown, T. A., Gati, J. S., Hughes, S. M., Nixon, P. L., Menon, R. S., Lomber, S. G. Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI. J. Vis. Exp. (84), e50872, doi:10.3791/50872 (2014).

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