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Editorial

June 2013: This Month in JoVE

Published: June 3, 2013 doi: 10.3791/5090
1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production

Summary

Here are some highlights from the June 2013 Issue of Journal of Visualized Experiments (JoVE).

Protocol

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta Using the Ex vivo Human Placental Perfusion Model

Stefanie Grafmüller1,2,3, Pius Manser2, Harald F. Krug2, Peter Wick2, Ursula von Mandach1

1Department of Obstetrics, Perinatal Pharmacology, University Hospital Zurich, 2Laboratory for Materials - Biology Interactions, EMPA Swiss Federal Laboratories for Materials Testing and Research, 3Graduate School for Cellular and Biomedical Sciences, University of Bern

The ex vivo dual recirculating human placental perfusion model can be used to investigate the transfer of xenobiotics and nanoparticles across the human placenta. In this video protocol we describe the equipment and techniques required for a successful execution of a placenta perfusion.

Generation of Human Cardiomyocytes: a Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Elisa Di Pasquale1,2, Belle Song1, Gianluigi Condorelli1

1Humanitas Clinical and Research Center, Italy, 2Institute of Genetic and Biomedical Research (IRGB), National Research Council (CNR)

Pluripotent stem cells, either embryonic or induced pluripotent stem (iPS) cells, constitute a valuable source of human differentiated cells, including cardiomyocytes. Here, we will focus on cardiac induction of iPS cells, showing how to use them to obtain functional human cardiomyocytes through an embryoid bodies-based protocol.

Shallow Water (Paddling) Variants of Water Maze Tests in Mice

Robert M.J. Deacon

Department of Experimental Psychology, University of Oxford

Mice can swim, but many strains appear to find this activity stressful. To overcome this problem mazes have been devised where escape from shallow water is used to motivate behaviour. These have been demonstrated to support learning at least as good as the traditional and widely used Morris water maze.

Functional Magnetic Resonance Imaging (fMRI) with Auditory Stimulation in Songbirds

Lisbeth Van Ruijssevelt, Geert De Groof, Anne Van der Kant, Colline Poirier, Johan Van Audekerke, Marleen Verhoye, Annemie Van der Linden

Bio-Imaging Lab, University of Antwerp

This article shows an optimized procedure for imaging of the neural substrates of auditory stimulation in the songbird brain using functional Magnetic Resonance Imaging (fMRI). It describes the preparation of the sound stimuli, the positioning of the subject and the acquisition and subsequent analysis of the fMRI data.

Perceptual and Category Processing of the Uncanny Valley Hypothesis' Dimension of Human Likeness: Some Methodological Issues

Marcus Cheetham, Lutz Jancke

Department of Neuropsychology, University of Zurich

Investigation of the Uncanny Valley Hypothesis and affective experience requires an understanding of the hypothesis' dimension of human likeness (DHL). This protocol allows representation of the DHL and examination of categorical perception. Use of the same stimuli and fMRI to distinguish brain regions responsive to physical and category change is illustrated.Investigation of the Uncanny Valley Hypothesis and affective experience requires an understanding of the hypothesis' dimension of human likeness (DHL). This protocol allows representation of the DHL and examination of categorical perception. Use of the same stimuli and fMRI to distinguish brain regions responsive to physical and category change is illustrated.

How to Detect Amygdala Activity with Magnetoencephalography using Source Imaging

Nicholas L. Balderston1, Douglas H. Schultz1, Sylvain Baillet2,3, Fred J. Helmstetter1,3

1Department of Psychology, University of Wisconsin-Milwaukee, 2McConnell Brain Imaging Centre, Montreal Neurological Institute, McGill University, 3Department of Neurology, Medical College of Wisconsin

This article describes how to record amygdala activity with magnetoencephalography (MEG). In addition this article will describe how to conduct trace fear conditioning without awareness, a task that activates the amygdala. It will cover 3 topics: 1) Designing a trace conditioning paradigm using backward masking to manipulate awareness. 2) Recording brain activity during the task using magnetoencephalography. 3) Using source imaging to recover signal from subcortical structures.

Disclosures

No conflicts of interest declared.

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June 2013: This Month in JoVE
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Chao, W., Kolski-Andreaco, A. JuneMore

Chao, W., Kolski-Andreaco, A. June 2013: This Month in JoVE. J. Vis. Exp. (76), e5090, doi:10.3791/5090 (2013).

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