Materials
Name | Company | Catalog Number | Comments |
2-Mercaptoethanol | Sigma | M3148 | |
50 mm Sterilin Petri Dish | Appleton Woods | SC265 | |
90 mm Sterilin Petri Dish | Appleton Woods | SC260 | |
Adhesive slides | Surgipath | 00202 | |
Anti-mouse CD31 eFluor 450 | eBioscience | 48-0311 | |
Anti-mouse CD4 Alexa 647 | eBioscience | 51-0041 | |
Anti-mouse CD45 PerCP-Cy5.5 | eBioscience | 45-0451 | |
Anti-mouse IgM Rhodamine Red | Stratech | 715-296-020-JIR | |
Anti-mouse Podoplanin PE/Cy7 | Biolegend | 127411 | |
Anti-mouse Podoplanin purified | eBioscience | 14-5381 | |
Collagenase D | Roche | 11088858001 | |
DMEM Medium | Sigma | D5671 | |
DNase I | Sigma | DN25-1G | |
Dumont #5 Forceps Inox Biologie | FST | 11252-20 | Extra fine forceps for embryonic and newborn dissections |
EDTA solution 0.5 M | Sigma | E7889 | |
ERTR-7 | Biogenesis | ||
Fetal bovine serum | Sigma | F9665 | |
Hardened fine iris scissors straight 11 cm | FST | 14090-11 | Small scissors for dissection |
HEPES solution | Sigma | H0887 | |
Isopore Membrane Filters 0.8 μm ATTP | Millipore | ATTPO 1300 | |
L-Glutamine solution | Sigma | G7513 | |
MEM Nonessential Amino Acid Solution (100x) | Sigma | M7145 | |
O.C.T. Compound | Tissue-Tek | 4583 | |
Penicillin-Streptomycin | Sigma | P4458 | |
Plastic box with lid | Watkins and Doncaster | E6052 | Sandwich box for in vitro organ culture. Drill two holes in the lid to allow gas exchange in the CO2 incubator. |
RPMI 1640 Medium | Sigma | R0883 | |
Sponges Vulkan Underwrap | Patterson Medical | 004383 | |
Stereomicroscope | Leica | LEICA MZ95 | Dissecting microscope with zoom |
Thermomixer | Eppendorf | 5436 | |
Vectashield Mounting Medium with DAPI | Vector Laboratories | H-1200 |
References
- Mueller, S. N., Germain, R. N. Stromal cell contributions to the homeostasis and functionality of the immune system. Nat. Rev. Immunol. 9, 618-629 (2009).
- Roozendaal, R., Mebius, R. E. Stromal cell-immune cell interactions. Annu. Rev. Immunol. 29, 23-43 (2011).
- Turley, S. J., Fletcher, A. L., Elpek, K. G. The stromal and haematopoietic antigen-presenting cells that reside in secondary lymphoid organs. Nat. Rev. Immunol. 10, 813-825 (2010).
- Katakai, T., et al. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs. J. Immunol. 181, 6189-6200 (2008).
- Link, A., et al. Fibroblastic reticular cells in lymph nodes regulate the homeostasis of naive T cells. Nat. Immunol. 8, 1255-1265 (2007).
- Benezech, C., et al. Ontogeny of stromal organizer cells during lymph node development. J. Immunol. 184, 4521-4530 (2010).
- Cupedo, T., et al. Presumptive lymph node organizers are differentially represented in developing mesenteric and peripheral nodes. J. Immunol. 173, 2968-2975 (2004).
- Avan de Pavert, S., Mebius, R. E. New insights into the development of lymphoid tissues. Nat. Rev. Immunol. 10, 664-674 (2010).
- Vondenhoff, M. F., et al. LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen. J. Immunol. 182, 5439-5445 (2009).
- Dougall, W. C., et al. RANK is essential for osteoclast and lymph node development. Genes Dev. 13, 2412-2424 (1999).
- Kim, D., et al. Regulation of peripheral lymph node genesis by the tumor necrosis factor family member TRANCE. J. Exp. Med. 192, 1467-1478 (2000).
- Carragher, D., et al. A stroma-derived defect in NF-kappaB2-/- mice causes impaired lymph node development and lymphocyte recruitment. J. Immunol. 173, 2271-2279 (2004).
- White, A., et al. Lymphotoxin a-dependent and -independent signals regulate stromal organizer cell homeostasis during lymph node organogenesis. Blood. 110, 1950-1959 (2007).
- Benezech, C., et al. Lymphotoxin-beta receptor signaling through NF-kappaB2-RelB pathway reprograms adipocyte precursors as lymph node stromal cells. Immunity. 37, 721-734 (2012).
- Anderson, G., Jenkinson, E. J., Moore, N. C., Owen, J. J. MHC class II-positive epithelium and mesenchyme cells are both required for T-cell development in the thymus. Nature. 362, 70-73 (1993).
- Szot, G. L., Koudria, P., Bluestone, J. A. Transplantation of pancreatic islets into the kidney capsule of diabetic mice. J. Vis. Exp. (9), e404 (2007).
- Krautler, N. J., et al. Follicular dendritic cells emerge from ubiquitous perivascular precursors. Cell. 150, 194-206 (2012).