July 2013: This Month in JoVE

Summary

Here are some highlights from the July 2013 Issue of Journal of Visualized Experiments (JoVE).

Protocol

The Resident-intruder Paradigm: A Standardized Test for Aggression, Violence and Social Stress

Jaap M. Koolhaas¹, Caroline M. Coppens¹, Sietse F. de Boer¹, Bauke Buwalda¹, Peter Meerlo¹, Paul J.A. Timmermans²

¹Department of Behavioral Physiology, Center for Behavior and Neurosciences, University Groningen, ²Radboud University Nijmegen

This video shows the resident-intruder paradigm in rats. This test is a standardized method to measure offensive aggression, defensive behavior and violence in a semi natural setting. The use of the paradigm for social stress experiments is explained as well.

Designing a Bio-responsive Robot from DNA Origami

Eldad Ben-Ishay, Almogit Abu-Horowitz, Ido Bachelet

Faculty of Life Sciences and the Institute for Nanotechnology & Advanced Materials, Bar-Ilan University

DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here we describe how DNA origami can be utilized to design a robotic robot capable of sensing biological cues and responding by shape shifting, subsequently relayed to a desired effect.

The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis

Rishu Gupta^1,2, Maya Debbaneh^2,3, Daniel Butler^2,4, Monica Huynh^2,5, Ethan Levin², Argentina Leon², John Koo², Wilson Liao²

¹Keck School of Medicine, University of Southern California, ²Psoriasis and Skin Treatment Center Dermatology, University of California, San Francisco, ³University of California Irvine School of Medicine, ⁴University of Arizona College of Medicine, ⁵Chicago College of Osteopathic Medicine

Psoriasis is a chronic, immune-mediated inflammatory skin disease. The Goeckerman regimen, formulated for the treatment of psoriasis, consists of exposure to ultraviolet B (UVB) light and application of crude coal tar (CCT). The following protocol is for the administration of Goeckerman therapy for the treatment of moderate-to-severe psoriasis.

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins

Kahlin Leuzinger*, Matthew Dent*, Jonathan Hurtado, Jake Stahnke, Huafang Lai, Xiaohong Zhou, Qiang Chen

The College of Technology and Innovation, Center for Infectious Disease and Vaccinology, The Biodesign Institute, Arizona State University

Plants offer a novel system for the production of pharmaceutical proteins on a commercial scale that is more scalable, cost-efficient and safe than current expression paradigms. In this study, we report a simple and convenient, yet scalable approach to introduce target-gene containing Agrobacterium tumefaciens into plants for protein transient expression.

Force Measurement During Contraction to Assess Muscle Function in Zebrafish Larvae

Darcée D. Sloboda¹, Dennis R. Claflin^1,2, James J. Dowling³, Susan V. Brooks^1,4

¹Department of Biomedical Engineering, University of Michigan, ²Department of Surgery, Section of Plastic Surgery, University of Michigan, ³Departments of Pediatrics and Neurology, University of Michigan, ⁴Department of Molecular and Integrative Physiology, University of Michigan

Force measurements can be used to demonstrate changes in muscle function due to development, injury, disease, treatment or chemical toxicity. In this video, we demonstrate a method to measure force during a maximal contraction of zebrafish larval trunk muscle.