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Bioengineering

PLGA-Nanopartikel durch Einzel-oder Doppel-Emulsion mit Vitamin E-TPGS gegründet

Published: December 27, 2013 doi: 10.3791/51015

Materials

Name Company Catalog Number Comments
Poly(lactic co-glycolic acid)  (PLGA) Lactel B6010-2P Inherent Viscosity: 0.55-0.75 dl/g
Ethyl acetate (EtAc) Sigma 270989
Methylene chloride (DCM)
Vitamin E TPGS Sigma 57668
Poly(vinyl alcohol) 87-89% hydrolized, 30,000-70,000 Da
Equipment
Sonic Dismembrator Fisher Scientific Model 705 700 W (Ultrasonicator)
Sonicator tip Fisher Scientific 1/8 in
Vortexer VWR 58816-121
Multiposition stirrer Corning MP5I
Ultracentrifuge Beckman-Coulter L8-80M
Fixed angle rotor Beckman-Coulter 50.2TI
Water bath sonicator Fisher Scientific FS30
Lyophilizer Millrock BT85

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References

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PLGA-Nanopartikel durch Einzel-oder Doppel-Emulsion mit Vitamin E-TPGS gegründet
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Cite this Article

McCall, R. L., Sirianni, R. W. PLGAMore

McCall, R. L., Sirianni, R. W. PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS. J. Vis. Exp. (82), e51015, doi:10.3791/51015 (2013).

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