Materials
| Name | Company | Catalog Number | Comments |
| EasySep Human CD4+ T Cell Enrichment Kit | Stem Cell | 19052 | |
| EasySep Human CD8+ T Cell Enrichment Kit | Stem Cell | 19053 | |
| CellTrace CFSE Cell Proliferation Kit - For Flow Cytometry | Invitrogen | C34554 | |
| Far Red LIVE/DEAD fixable dead cell | Invitrogen | L10120 | |
| Alexa Fluor 700 Mouse Anti-Human CD3 | BD | 557943 | |
| Brilliant Violet 650 Anti-Human CD4 Antibody | Biolegend | 317435 | |
| PerCP anti-human CD8 Antibody | Biolegend | 344707 | |
| RPMI 1640 Medium 1x | VWR | 10-040-CV | |
| Penicillin-Streptomycin 100x Solution | Invitrogen | 15070063 | |
| Fetal Bovine Serum - Gold, Heat Inactivated | PAA | A15-252 | |
| 96-well plates; Treated; U-bottom; with lid | BD Falcon | 353077 | |
| 25 ml sterile polystyrene, individually wrapped reservoirs | USA Scientific | 2321-2230 | |
| 96-well U-bottom, sterile 2 ml deep well plates | Thermo Scientific | 275743 | |
| Human IL-2 (v126), research grade | Miltenyi Biotec | 130-093-096 | |
| RoboSep Buffer, separation buffer | Stem cell | 20104 | |
| 15 ml Polypropylene Conical Tube | BD Falcon | 352097 | |
| 14 ml round bottom tube, polypropylene | BD Falcon | 352059 | |
| The Big Easy EasySep Magnet | Stem cell | 18001 | |
| PBS 1x | VWR | 21-040-CV | |
| 96-well; V-bottom plate with lid | Fisher | 12-565-481 | |
| Formaldehyde solution, ACS reagent, 37 wt % in H2O | Sigma-Aldrich | 252549-500ML | |
| 1.5 ml screw cap microtube, conical | Sarstedt | 72.692.005 |
References
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