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方法对纸浆厂污水流和培养微生物的生物降解能力的表征促进微生物生长

Published: December 12, 2013 doi: 10.3791/51373

Abstract

硫酸盐处理应用于木屑对木质素从多糖中分离木素纤维素纸浆,将产生一个高质量的纸张。黑液是由牛皮纸工艺,具有潜在的下游生物转化产生的制浆废水。然而,木质纤维素资源,这占了大部分内黑液可用的有机碳,以及其碱性pH存在挑战,这浪费材料的微生物降解及其化学衍生物的顽抗性质。方法黑液微生物生长的收集和修改的目的是利用这种纸浆废液中的木质素,有机酸和多糖降解副产物转化成有价值的化学物质。介绍了木质纤维素的提取技术制备木质纤维素底物的增长理解为培养微生物的代谢能力,提供了一个可重复的方法。使用燃气通道的romatography - 质谱法使之从微生物对制浆废水增长所带来的发酵产物的鉴定和定量。这些方法一起使用时可以促进微生物的潜在生产发酵产品,将提供更大的价值,制浆系统,减少污水垃圾,从而增加潜在纸铣利润,并提供用于黑液的其他用途的代谢活动的决心。

Materials

Name Company Catalog Number Comments
1,4-Dioxane (Certified ACS) Fisher D111 Flammable, preoxidizable chemical
Black Liquor Department of Forest Biomaterials at North Carolina State University N/A pH 12.72, 13.67% solids
Ethanol (microbiology grade) Fisher BP2818
Ethyl Acetate (ACS reagent grade) EMD EX0240-9 Flammable
Glacial acetic acid (Certified ACS) EMD AX0073 Corrosive
Guaiacol Sigma Aldrich PHR1136 Harmful by ingestion, corrosive
HP-5 capillary column Agilent 19091J-577 60 m x 0.18 mm internal diameter, 0.18 μm thickness
Hydrochloric acid (certified ACS) EMD HX0603P
N,O-Bis(trimethylsilyl)trifluoroacetamide with trimethylcholorsilane 99:1% (BSTFA) Fluka 15238 Flammable, causes skin burns and eye damage with contact
Na2SO4 (FCC grade) VWR BDH8026
NaClO2 (80%) Sigma Aldrich 244155 Flammable, toxic  
NaOH (certified ACS) EMD 1.06498.1000 Corrosive
Alkacid pH test ribbons Fisher A979
Phosphoric Acid (certified ACS) Fisher A242
Polaris Q mass spectrometer  Thermo Electron Corporation
Pyridine (99%) Alfa Aesar A12005 Flammable, toxic in contact with skin
Switchgrass Cherry Research Farm Goldsboro, NC N/A Harvested August 2011
Thermo Finnigan trace gas chromatograph Thermo Electron Corporation
Whatman no. 1 filter paper Whatman 1001-150

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References

  1. Tejado, A., Pena, C., Labidi, J., Echeverria, J. M., Mondragon, I. Physico-chemical characterization of lignin from different sources for use in phenol-formaldehyde resin synthesis. Bioresource Technol. 98, 1655-1663 (2007).
  2. Brannvall, E. Overview of pulp and paper processes. Pulp and paper chemistry and technology. Elk, M., Gellerstedt, G., Henriksson, G. 2, De Gruyter. 1-12 (2009).
  3. Biermann, C. J. Pulping fundamentals. Handbook of pulping and papermaking. , 2nd edition, Academic Press INC. 55-100 (1996).
  4. Sjöström, E. Wood chemistry: fundamentals and applications. , 2nd, San Deigo Academic Press. (1924).
  5. Philbrook, A., Alissandratos, A., Easton, C. J. Biochemical processes for generating fuels and commodity chemicals from lignocellulosic biomass. Environ. Biotechnol. , 39-63 (2013).
  6. Sanchez, R., Ferrer, A., Serrano, L., Toledano, A., Labidi, J., Rodriguez, A. Hesperaloafunifera as a raw material for integral utilization of its components. BioResources. 6 (1), 3-21 (2011).
  7. Font, X., Caminal, G., Gabarrel, X., Romero, S., Vicent, M. T. Black liquor detoxification by laccase of Trametesversicolorpellets. J. Chemical Tech. Biotech. 78, 548-554 (2003).
  8. Zimmerman, W. Degradation of lignin by bacteria. J. Biotechnol. 13, 119-130 (1990).
  9. Ahamad, M., Taylor, C. R., Pink, D., Burton, K., Eastwood, D., Bending, G. R., Bugg, T. D. H. Development of novel assay for lignin degradation: comparative analysis of bacterial and fungal lignin degraders. Mol. Biosystems. 6, 815-821 (2010).
  10. Ramchandra, M., Crawford, D. L., Hertel, G. Characterization of an extracellular lignin peroxidase of the lignocellulolyticactinomycete Streptomyces viridosporus. Appl. Environ. Micro. 54, 3057-3063 (1988).
  11. Mishra, M., Thakur, I. S. Microorganisms in environmental management: microbes and the environment. Satyanarayana, T., Johri, B. N., Prakash, A. , Springer. (2012).
  12. Karaaslan, A. M., Tshabalala, M. A., Wood Bushcle-Diller, G. hemicellulose chitosan-based semi-interpenetrating network hydrogels: mechanical, swelling and controlled drug release properties. BioResources. 5 (2), 1036-1054 (2010).
  13. Lech, K., Brent, R. Short protocols in molecular biology. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Kevin, S. , 2nd, John Wiley & Sons. (1992).
  14. Raj, A., Krishna Reddy, M. M., Chandra, R. Identification of low molecular weight aromatic compounds by gas chromatography-mass spectrometry (GC-MS) from kraft lignin degradation by three Bacillus sp. Int. Biodeterior. Biodegradation. 59, 292-296 (2007).
  15. Chandra, R., Raj, A., Purohit, H. J., Kapley, A. Biodegradation of kraft lignin by three pure and mixed aerobic bacterial cultures isolated from pulp and paper sludge. Proceeding of the 25th international symposium on toxicology environmental and occupational health, , (2005).
  16. Chen, Y., Chai, L., Tang, C., Yang, Z., Zheng, Y., Shi, Y., Zhang, H. Kraft lignin biodegradation by Novosphingobium sp. B-7 and analysis of the degradation process. Bioresource Technol. 123, 682-685 (2012).
  17. Bandounas, L., Wierckx, N. J., de Winde, J. H., Ruijssenaars, H. J. Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential. BMC Biotechnol. 11 (94), (2011).
  18. Chandra, R., Abhishek, A., Sankhwar, M. Bacterial decolorization and detoxification of black liquor from rayon grade pulp manufacturing industry and detection of their metabolic products. Bioresource Technol. 102, 6429-6436 (2011).
  19. Gellerdtedt, G. Chemistry of Bleaching of Chemical Pulp. Pulp and Paper Chemistry and Technology. Ek, M., Gellerstedt, G., Henriksson, G. 2, De Gruyter. 201-237 (2009).
  20. Kringstad, K. P., Lindstrom, K. Spent liquors from pulp bleaching. Environ. Sci. Tech. 18, 236-247 (1984).
方法对纸浆厂污水流和培养微生物的生物降解能力的表征促进微生物生长
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Cite this Article

Mathews, S. L., Ayoub, A. S.,More

Mathews, S. L., Ayoub, A. S., Pawlak, J., Grunden, A. M. Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes. J. Vis. Exp. (82), e51373, doi:10.3791/51373 (2013).

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